NYMU Taipei/Primer List
From 2007.igem.org
(Difference between revisions)
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|Reverse sequencing primer. | |Reverse sequencing primer. | ||
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* [http://www.mdbio.com.tw/pfu.htm pfu primer] | * [http://www.mdbio.com.tw/pfu.htm pfu primer] | ||
Revision as of 12:03, 20 August 2007
| Name | Sequence | Length | Tm (C) | Restriction site | Target length | Description |
|---|---|---|---|---|---|---|
| M_INS_A 5' | GTTTCTTCGAATTCGCGGCCGCTTCTAGATGGGCATTGTAGATCAGTGCTG | 51 bp | 56.85 | EcoRI, XbaI | 63 bp | Potential dimer formation! |
| M_INS_A 3' | GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGTTGCAGTAGTTCTCCAGCT | 55 bp | 54.74 | SpeI, PstI | 63 bp | Potential dimer formation! |
| M_INS_B 5' | GTTTCTTCGAATTCGCGGCCGCTTCTAGATGTTTGTCAAGCAGCACCTTTGT | 52 bp | 60.85 | EcoRI, XbaI | 90 bp | |
| M_INS_B 3' | GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGGACATGGGTGTGTAGAAGA | 55 bp | 55.86 | SpeI, PstI | 90 bp | |
| RcsC 5' | GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAAATACCTTGCTTCTTTTCG | 51 bp | 55.92 | EcoRI, XbaI | 1396 bp | |
| RcsC 3'-2 | GGGGTATACCAACGACTCTTCCATCTTCAC | 29 bp | 59.63 | NdeI | 1396 bp | Two extra adenosine was added to the 5' side of the NdeI cutting site. |
| VF2 | tgccacctgacgtctaagaa | 20 bp | 59.44 | Forward sequencing primer. | ||
| VR | attaccgcctttgagtgagc | 20 bp | 59.34 | Reverse sequencing primer. |
