NYMU Taipei/phptest

From 2007.igem.org

(Difference between revisions)
Line 17: Line 17:
<form name=form1>
<form name=form1>
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     <INPUT type="text" name="insert_size" > (bp) size of insert part (SI)<BR>
+
     <INPUT type="text" name="insert_size" > size (bp) of insert part (SI)<BR>
-
     <INPUT type="text" name="vector_size" > (bp) size of vector part (SV)<BR>
+
     <INPUT type="text" name="vector_size" > size (bp) of vector part (SV)<BR>
-
     <INPUT type="text" name="I" > (ug/uL) concentration of insert (CI)<BR>
+
     <INPUT type="text" name="I" > concentration (ug/uL) of insert (CI)<BR>
-
     <INPUT type="text" name="V" > (ug/uL) concentration of vector (CV)<BR>
+
     <INPUT type="text" name="V" > concentration (ug/uL) of vector (CV)<BR>
     <INPUT type="text" name="X" > volume (uL) of insert for gel separation (VI)<BR>
     <INPUT type="text" name="X" > volume (uL) of insert for gel separation (VI)<BR>
     <INPUT type="text" name="L" > volume (uL) of insert for  ligation (L)<BR>
     <INPUT type="text" name="L" > volume (uL) of insert for  ligation (L)<BR>
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----
----
* formula
* formula
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** (1) 300 * SI * 3 * 10 / SV = CI * X * L /50
+
** (1) 300 * SI * 3 * 10 (損失因子) / SV = CI * X * L /50
** (2) 300 ng = CV (ng/uL) * VV (uL), VV = 300/CV
** (2) 300 ng = CV (ng/uL) * VV (uL), VV = 300/CV
** VV (uL) is volume of vector
** VV (uL) is volume of vector
** CV (ng/uL) is concentration of vector
** CV (ng/uL) is concentration of vector

Revision as of 07:00, 16 September 2007

size (bp) of insert part (SI)
size (bp) of vector part (SV)
concentration (ug/uL) of insert (CI)
concentration (ug/uL) of vector (CV)
volume (uL) of insert for gel separation (VI)
volume (uL) of insert for ligation (L)


  • total digestion volume is 20 uL (typical)
    • enzyme 1 and enzyme 2 (1uL * 2)
    • 10X buffer (2uL)
    • BSA (2uL)
    • plasmid + ddH2O (14uL)

  • total gel separation volume is 50 uL
    • vector weight is 300 ng

  • formula
    • (1) 300 * SI * 3 * 10 (損失因子) / SV = CI * X * L /50
    • (2) 300 ng = CV (ng/uL) * VV (uL), VV = 300/CV
    • VV (uL) is volume of vector
    • CV (ng/uL) is concentration of vector