USTC/Demonstration

From 2007.igem.org

< USTC(Difference between revisions)
m (Actual System)
(Actual System)
 
(13 intermediate revisions not shown)
Line 1: Line 1:
An actual demonstration is determined to show the extensibility of our method.  
An actual demonstration is determined to show the extensibility of our method.  
-
This demo system  
+
 
-
is designed as simple as possible, without any "cool" logic function;  
+
 
-
includes all the three logic gates, which constitute a three-level logic circuit;  
+
This demo system
-
shows that wires can cross and branch off (without interference);  
+
* is designed as simple as possible, without any "cool" logic function;  
-
is loaded on two plasmids, pSB1A3-I732998 and pSB1A3-I732999 ,and practically transformed into Top10 strain;  
+
* includes all the three logic gates, which constitute a three-level logic circuit;  
-
accepts aTc and AHL signals as inputs;  
+
* shows that wires can cross and branch off (without interference);  
-
produces RFP and GFP signals as outputs;  
+
* is loaded on two plasmids, [[http://partsregistry.org/Part:BBa_I732923 pSB1A3-I732923]] and [[http://partsregistry.org/Part:BBa_I732925 pSB3K5-I732925]] ,and practically transformed into Top10 strain;  
-
can be "reset" by IPTG signal (i.e. both of the outputs are lightened when IPTG added in);  
+
 
-
is expected to put out the results that accord with the truth value shown in Figure 2.  
+
* accepts aTc and AHL signals as inputs;  
 +
* produces RFP and GFP signals as outputs;  
 +
* can be "reset" by IPTG signal (i.e. both of the outputs are lightened when IPTG added in);  
 +
* is expected to put out the results that accord with the truth value shown in Figure 2.  
Line 20: Line 23:
<br style="clear:both;">
<br style="clear:both;">
-
We can see from this truth table that IPTG might result in both outputs lightened no matter aTc or AHL exists or not. (It is just like the response "8888..." on the screen when you reset one of your digital equipment, for example, a calculator.)
+
We can see from this truth table that IPTG might result in both of the outputs being lightened no matter whether aTc or AHL exists or not. (It is just like the response "8888..." on the screen when you reset some of your electrical apparatus, for example, a calculator.)
=Actual System=
=Actual System=
Line 26: Line 29:
<br style="clear:both;">
<br style="clear:both;">
-
The three parts on the left are loaded on pSB1A3-I732998, and the rest two parts are loaded on pSB1A3-I732999. We've built up this system in one kind of TOP10 E.coli, but it seems that it cannot grow stably when aTc exists. (We have to use aTc at a high concentration for the sake of the high expression of LuxR.) The very final results here may not be able to show on time by Oct. 26, but we have continually been trying our best to screen out such a monoclonal strain. You will get the results in the coming presentation...
+
The three parts on the left are loaded on [[http://partsregistry.org/Part:BBa_I732923 pSB1A3-I732923]], and the rest two parts are loaded on [[http://partsregistry.org/Part:BBa_I732925 pSB3K5-I732925]]. We've built up this system in one kind of TOP10 ''E.coli'', but it seems that it cannot grow stably when concentrated aTc exists. (We have to use aTc at a high concentration for counteracting the high expression of tetR, but concentrated aTc shows some bacteriostatic activity like Tc(TetraCycline).) We may not be able to send out the very final results here exactly by Oct. 26, but we have continually been trying our best to screen out such a monoclonal strain that is not influenced by concentrated aTc. You will get the results in the coming presentation...
<br>
<br>
<br>
<br>

Latest revision as of 04:33, 27 October 2007

An actual demonstration is determined to show the extensibility of our method.


This demo system

  • accepts aTc and AHL signals as inputs;
  • produces RFP and GFP signals as outputs;
  • can be "reset" by IPTG signal (i.e. both of the outputs are lightened when IPTG added in);
  • is expected to put out the results that accord with the truth value shown in Figure 2.


Logic Abstract of the Demo

Figure 1 The logic diagram of the demo system.


Figure 2 The truth table of the demo system.


We can see from this truth table that IPTG might result in both of the outputs being lightened no matter whether aTc or AHL exists or not. (It is just like the response "8888..." on the screen when you reset some of your electrical apparatus, for example, a calculator.)

Actual System

Figure 3 The signal pathway of the demo system.


The three parts on the left are loaded on http://partsregistry.org/Part:BBa_I732923 pSB1A3-I732923, and the rest two parts are loaded on http://partsregistry.org/Part:BBa_I732925 pSB3K5-I732925. We've built up this system in one kind of TOP10 E.coli, but it seems that it cannot grow stably when concentrated aTc exists. (We have to use aTc at a high concentration for counteracting the high expression of tetR, but concentrated aTc shows some bacteriostatic activity like Tc(TetraCycline).) We may not be able to send out the very final results here exactly by Oct. 26, but we have continually been trying our best to screen out such a monoclonal strain that is not influenced by concentrated aTc. You will get the results in the coming presentation...