Week 10
From 2007.igem.org
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*There are colonies for every ligations. | *There are colonies for every ligations. | ||
*Meeting. | *Meeting. | ||
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::'''09/05/07''' | ::'''09/05/07''' | ||
Miniprep for: | Miniprep for: | ||
- | -I763016+I763020(I763019); | + | - [http://partsregistry.org/Part:BBa_I763016 I763016]+ [http://partsregistry.org/Part:BBa_I763020 I763020] ([http://partsregistry.org/Part:BBa_I763019 I763019]); |
- | -I763025+B0015(I763026); | + | - [http://partsregistry.org/Part:BBa_I763025 I763025]+ [http://partsregistry.org/Part:BBa_B0015 B0015] ([http://partsregistry.org/Part:BBa_I763026 I763026]); |
- | -I763024+B0015(I763030); | + | - [http://partsregistry.org/Part:BBa_I763024 I763024]+ [http://partsregistry.org/Part:BBa_B0015 B0015] ([http://partsregistry.org/Part:BBa_I763030 I763030]); |
- | -R0051+I763020(I763011); | + | - [http://partsregistry.org/Part:BBa_R0051 R0051]+ [http://partsregistry.org/Part:BBa_I763020 I763020] ([http://partsregistry.org/Part:BBa_I763011 I763011]); |
- | -I763009+P0412(I763021); | + | - [http://partsregistry.org/Part:BBa_I763009 I763009]+ [http://partsregistry.org/Part:BBa_P0412 P0412] ([http://partsregistry.org/Part:BBa_I763021 I763021]); |
- | -I763009+S03520(I763022); | + | - [http://partsregistry.org/Part:BBa_I763009 I763009]+ [http://partsregistry.org/Part:BBa_S03520 S03520]([http://partsregistry.org/Part:BBa_I763022 I763022]); |
*Digestion for: | *Digestion for: | ||
- | -I763019 with Eco/Xba; | + | - [http://partsregistry.org/Part:BBa_I763019 I763019] with Eco/Xba; |
- | -I763026 with Eco/Spe; | + | - [http://partsregistry.org/Part:BBa_I763026 I763026] with Eco/Spe; |
- | -I763030 with Eco/Spe; | + | - [http://partsregistry.org/Part:BBa_I763030 I763030] with Eco/Spe; |
- | -I763011 with Xba/Pst1; | + | - [http://partsregistry.org/Part:BBa_I763011 I763011] with Xba/Pst1; |
- | -R0051+I13507(I763007) with Xba/Pst1; | + | - [http://partsregistry.org/Part:BBa_R0051 R0051]+ [http://partsregistry.org/Part:BBa_I13507 I13507]([http://partsregistry.org/Part:BBa_I763007 I763007]) with Xba/Pst1; |
- | -I763021 with Eco/Spe; | + | - [http://partsregistry.org/Part:BBa_I763021 I763021] with Eco/Spe; |
- | *Control digestion for I763019 with Eco/Pst1; | + | *Control digestion for [http://partsregistry.org/Part:BBa_I763019 I763019] with Eco/Pst1; |
*Band extraction for | *Band extraction for | ||
- | - | + | - [http://partsregistry.org/Part:BBa_I763019 I763019]; |
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- | - | + | - [http://partsregistry.org/Part:BBa_I763026 I763026]; |
- | - | + | - [http://partsregistry.org/Part:BBa_I763011 I763011]; |
- | - | + | - [http://partsregistry.org/Part:BBa_R0051 R0051]+ [http://partsregistry.org/Part:BBa_I13507 I13507] ([http://partsregistry.org/Part:BBa_I763007I763007]); |
*Band extraction for I763019 (digested with Xba/Spe and with Xba/Pst1), I763026; | *Band extraction for I763019 (digested with Xba/Spe and with Xba/Pst1), I763026; |
Revision as of 20:46, 7 September 2007
- 09/03/07
- Miniprep for:
-[http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_S0100 S0100];
-[http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_S03520 S03520];
-[http://partsregistry.org/Part:BBa_I763005 I763005]+[http://partsregistry.org/Part:BBa_I763015 I763015];
-[http://partsregistry.org/Part:BBa_J52034 J52034]+[http://partsregistry.org/Part:BBa_E0022 E0022];
- Digestion for:
-[http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_S0100 S0100] with Eco/Spe;
-[http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_S03520 S03520] with Eco/Spe;
-[http://partsregistry.org/Part:BBa_I763005 I763005]+[http://partsregistry.org/Part:BBa_I763015 I763015] with Spe/Pst1;
-[http://partsregistry.org/Part:BBa_J52034 J52034]+[http://partsregistry.org/Part:BBa_E0022 E0022] with Spe/Pst1;
-[http://partsregistry.org/Part:BBa_B0015 B0015] with Eco/Xba;
- Band extraction;
- Ligation for:
-[http://partsregistry.org/Part:BBa_I763025 I763025]+[http://partsregistry.org/Part:BBa_B0015 B0015]([http://partsregistry.org/Part:BBa_I763026 I763026]);
-[http://partsregistry.org/Part:BBa_I763024 I763024] +[http://partsregistry.org/Part:BBa_B0015 B0015]([http://partsregistry.org/Part:BBa_I763030 I763030]);
-[http://partsregistry.org/Part:BBa_I763016 I763016]+[http://partsregistry.org/Part:BBa_I763020 I763020]([http://partsregistry.org/Part:BBa_I763019 I763019]);
-[http://partsregistry.org/Part:BBa_I763009 I763009]+[http://partsregistry.org/Part:BBa_P0412 P0412]([http://partsregistry.org/Part:BBa_I763021 I763021]);
-[http://partsregistry.org/Part:BBa_I763009 I763009]+[http://partsregistry.org/Part:BBa_S0100 S0100]([http://partsregistry.org/Part:BBa_I763023 I763023]);
-[http://partsregistry.org/Part:BBa_I763009 I763009]+[http://partsregistry.org/Part:BBa_S03520 S03520]([http://partsregistry.org/Part:BBa_I763022 I763022]);
-[http://partsregistry.org/Part:BBa_R0051 R0051]+[http://partsregistry.org/Part:BBa_I763020 I763020]([http://partsregistry.org/Part:BBa_I763011 I763011]);
- 09/04/07
- There are colonies for every ligations.
- Meeting.
- 09/05/07
Miniprep for:
- [http://partsregistry.org/Part:BBa_I763016 I763016]+ [http://partsregistry.org/Part:BBa_I763020 I763020] ([http://partsregistry.org/Part:BBa_I763019 I763019]);
- [http://partsregistry.org/Part:BBa_I763025 I763025]+ [http://partsregistry.org/Part:BBa_B0015 B0015] ([http://partsregistry.org/Part:BBa_I763026 I763026]);
- [http://partsregistry.org/Part:BBa_I763024 I763024]+ [http://partsregistry.org/Part:BBa_B0015 B0015] ([http://partsregistry.org/Part:BBa_I763030 I763030]);
- [http://partsregistry.org/Part:BBa_R0051 R0051]+ [http://partsregistry.org/Part:BBa_I763020 I763020] ([http://partsregistry.org/Part:BBa_I763011 I763011]);
- [http://partsregistry.org/Part:BBa_I763009 I763009]+ [http://partsregistry.org/Part:BBa_P0412 P0412] ([http://partsregistry.org/Part:BBa_I763021 I763021]);
- [http://partsregistry.org/Part:BBa_I763009 I763009]+ [http://partsregistry.org/Part:BBa_S03520 S03520]([http://partsregistry.org/Part:BBa_I763022 I763022]);
- Digestion for:
- [http://partsregistry.org/Part:BBa_I763019 I763019] with Eco/Xba;
- [http://partsregistry.org/Part:BBa_I763026 I763026] with Eco/Spe;
- [http://partsregistry.org/Part:BBa_I763030 I763030] with Eco/Spe;
- [http://partsregistry.org/Part:BBa_I763011 I763011] with Xba/Pst1;
- [http://partsregistry.org/Part:BBa_R0051 R0051]+ [http://partsregistry.org/Part:BBa_I13507 I13507]([http://partsregistry.org/Part:BBa_I763007 I763007]) with Xba/Pst1;
- [http://partsregistry.org/Part:BBa_I763021 I763021] with Eco/Spe;
- Control digestion for [http://partsregistry.org/Part:BBa_I763019 I763019] with Eco/Pst1;
- Band extraction for
- [http://partsregistry.org/Part:BBa_I763019 I763019];
- [http://partsregistry.org/Part:BBa_I763026 I763026];
- [http://partsregistry.org/Part:BBa_I763011 I763011];
- [http://partsregistry.org/Part:BBa_R0051 R0051]+ [http://partsregistry.org/Part:BBa_I13507 I13507] ([http://partsregistry.org/Part:BBa_I763007I763007]);
- Band extraction for I763019 (digested with Xba/Spe and with Xba/Pst1), I763026;
- Ligation O/N for:
-I763026+I763019(I763028);
-I763026+I763004(I763027);
- 09/06/07
Digestion for:
-I763019 with Eco/Xba;
-I763021 with Eco/Spe;
-I763026 with Eco/Spe.
- Band extraction from electrophoresis gel for I763019 and I763026.
- Ligation for I763027 with new extracts.
- Transformations for the new ligations.
- 09/07/07