Bologna University/Soc medium


1. To prepare 1 L of SOC medium dissolve in ultrapure water:
-Tryptone 4 g
-Yeast extract 1g
-1M NaCl 2 ml
-1M KCl 0.5 ml
-5M NaOH 200 μl to adjust the pH to 6.8
2. After autoclaving add 2 ml each of 2M Mg-salt (1M MgSO4 and 1M MgCl) and 2M glucose.