Melbourne/Making glycerol Stocksl


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  • Applications: Create a stock of recombinant bacteria that can be stored at -70C

  • Time to complete protocol: 5min
    • Lab time: 5 minutes
    • Waiting time: 0
  • Approximate cost of materials: $


Method from primary and secondary reagents

Primary & secondary Reagents Required including controls
Method including controls
  1. Add to a sterile Screw Top tube
    -800ul of overnight bacterial culture
    -600ul of glycerol
  2. Mix by inversion
  3. Immerse in liquid nitrogen and store in -70C freezer. Alternatively, cover the tube with dry ice and pour ethanol in the dry ice. Store immediately in -70C freezer.
Equipement Required