From 2007.igem.org

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• Applications: Create a stock of recombinant bacteria that can be stored at -70C

• Time to complete protocol: 5min
  • Lab time: 5 minutes
  • Waiting time: 0
• Approximate cost of materials: $

Contents 1 Method from primary and secondary reagents 1.1 Primary & secondary Reagents Required including controls 1.2 Method including controls 1.3 Equipement Required 1.4 References

Method from primary and secondary reagents

Primary & secondary Reagents Required including controls

• Overnight bacterial culture
• Glycerol
• Liquid Nitrogen or Dry Ice and Ethanol

Method including controls

1. Add to a sterile Screw Top tube
   -800ul of overnight bacterial culture
   -600ul of glycerol
2. Mix by inversion
3. Immerse in liquid nitrogen and store in -70C freezer. Alternatively, cover the tube with dry ice and pour ethanol in the dry ice. Store immediately in -70C freezer.

Equipement Required

• Screw Top tube
• Pipettes

References