Mini and Midi prep
Equipment and Reagents to Be Supplied by User
When working with chemicals, always wear a suitable lab coat, disposable gloves, and protective goggles. For more information, consult the appropriate material safety data sheets (MSDSs), available from the product supplier.
For all protocols:
■ Standard microbiological equipment for growing and harvesting bacteria (e.g., inoculating loop, culture tubes and flasks, 37°C shaking incubator, and centrifuge with rotor and tubes or bottles for harvesting cells)
■ QIArack or equivalent holder (see “Setup of QIAGEN-tips”, page 13)
■ 70% ethanol
■ Plasmid resuspension buffer (e.g., TE buffer, pH 8.0, or Tris·Cl, pH 8.5)
For QIAGEN Plasmid Mini Kit protocol:
■ 1.5 ml or 2 ml microcentrifuge tubes
For QIAGEN Plasmid Midi, Maxi, Mega, and Giga Kit protocols:
■ Centrifugation tubes or vessels with suitable capacity for the volumes specified in the appropriate protocol.
■ Refrigerated centrifuge capable of ≥20,000 x g with rotor for the appropriate centrifuge tubes or bottles