Tokyo/sunaba

From 2007.igem.org

砂場。


Homepage1.JPG

For detailed description of each section, plese click "more infromation"

1,Project summary

Concept& model: Bacterial society that follows Pareto's principle like ant society


TOP.JPG
(木賀)ここに文字を入れると左カラムが間延びするのでよくない。図に少し説明入れて、文章はクリックしたら出るようにするべきか。それとも2カラム制をあきらめる?In the worker ants, we can find true workers and idlers even though they have the same genome. In the abscence of the ture workers, a part of idlers become the true worker. We tried to construct such society of bacteria.

Requirements:
bistability and cell-cell communication

Genetic circuit with new part:


Tokyo genetic circuit s.jpg

Simulation:using parameters from our wet experiments


Tokyo simulation s2.jpg

Our construction parts are Here!!!

2,Works

Approach:We alternated approach of Wet and Dry.

2-0.Wet : Hybrid promoter

For our circuit, we developped the promoter that can receive two input,AHL & reperssor.


図 配列と棒グラフ

Hybrid.JPG

2-1.Dry : Formulation

We wrote the Formulation



Expression3-1.jpgAHLresponse2-2.jpg
Phaseplane3-1.jpgPhaseplane3-3.jpg

2-2.Wet : Assay

We decided the parameter of hill coefficients by the assay of AHL & IPTG

Hill AHL.JPG|Hill IPTG.JPG
ns=2.3 nb=2.7

2-3.Dry : Simulation

wet実験で求められたパラメータを用いて、集団が怠け者の細胞のみとなった場合に、一度不安定化して、続いて働き者と怠け者になれることをStocastic Simulationで確認した。このシステムの挙動に必要な他のパラメータ範囲が判明した。
(誰かの提案)最終日に動画と差し替え??
(木賀)はい、動画ではなくても、左カラムとは別の図にしましょう。


Tokyo simulation s2.jpg

2-4.Wet : Assay

Simulationを満たすための他のパラメータが現在のプラスミドで可能であるかを、大腸菌を用いた実験で確認した。

2-5.Future works

3,About our team


Students
Satoru Akama | Kenichiro Iwasaki | Hiroki Kawahara | Hajime Nakatani

Advisors: Last year's member
Syotarou Ayukawa | Akio Kobayashi

Instructors
Daisuke Kiga | Masayuki Yamamura | Masami Hagiya | Masahiko Uchiyama

Links sunaba

Homepage1.JPG


Project

Concept: Ant society and Pareto's principle
Model : Bacterial society that follows Pareto's principle Homepage3.jpg
Requirements: bistability and cell-cell communication

Experiments

Wet

Construction / Assay

Dry

Analysis / Simulation


Achievements

About our team


Students
Satoru Akama | Kenichiro Iwasaki | Hiroki Kawahara | Hajime Nakatani

Advisors: Last year's member
Syotarou Ayukawa | Akio Kobayashi

Instructors
Daisuke Kiga | Masayuki Yamamura | Masami Hagiya | Masahiko Uchiyama

Links sunaba Home http://2007.igem.org/Tokyo_Tech

sunaba2