Melbourne/Blue Photosensor Background
From 2007.igem.org
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====Preliminaries==== | ====Preliminaries==== | ||
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SopII - sensory rhodopsin II | SopII - sensory rhodopsin II | ||
[[Melbourne/Blue Photosensor Background/SopII/DNA|DNA]], | [[Melbourne/Blue Photosensor Background/SopII/DNA|DNA]], | ||
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* No IGEM restriction sites | * No IGEM restriction sites | ||
* HaeII site at bp - 168 | * HaeII site at bp - 168 | ||
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HtrII - sensory rhodopsin II transducer | HtrII - sensory rhodopsin II transducer | ||
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* No IGEM restriction sites | * No IGEM restriction sites | ||
* No useful restriction site | * No useful restriction site | ||
| + | |||
SopII/HtrII fusion | SopII/HtrII fusion | ||
Revision as of 11:20, 6 August 2007
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Preliminaries
SopII - sensory rhodopsin II DNA, AA [http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=search&term=sopII genbank]
- No IGEM restriction sites
- HaeII site at bp - 168
HtrII - sensory rhodopsin II transducer
DNA,
AA
[http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=3702851&ordinalpos=1&itool=EntrezSystem2.PEntrez.Gene.Gene_ResultsPanel.Gene_RVDocSum genbank]
- No IGEM restriction sites
- No useful restriction site
SopII/HtrII fusion
DNA,
AA (frame 2 in Translate)
[http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=search&term=sopII genbank]
- No IGEM restriction sites
- Use site from SopII (this is the one that will be used)
ComP - two-component sensor histidine kinase
DNA,
AA
[http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=search&term=sopII genbank]
SpeI (@927) deleted
HaeII (@1902) inserted
ComA - sensory rhodopsin II transducer
DNA,
AA
[http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=search&term=sopII genbank]
SpeI (@12) deleted
HaeII (@433) inserted
File:Seq align.jpg Media:Example.ogg
