Melbourne/primary Restriction enzymes

From 2007.igem.org

(Difference between revisions)
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!Enzyme
!Enzyme
!Cut site
!Cut site
 +
!100% buffers
!Cost
!Cost
!No of U
!No of U
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!PstI
!PstI
|5CTGCA^G3
|5CTGCA^G3
 +
|
|US$58
|US$58
|10,000 @ 20,000U/ml
|10,000 @ 20,000U/ml
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!EcoRI
!EcoRI
|5G^AATTC3
|5G^AATTC3
 +
|
|US$53
|US$53
|10,000 @ 20,000U/ml
|10,000 @ 20,000U/ml
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!HaeII
!HaeII
|5RGCGC^Y3
|5RGCGC^Y3
 +
|
|US$53
|US$53
|2,000U @ 20,000U/ml
|2,000U @ 20,000U/ml
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!SalI
!SalI
|5G^TCGAC3
|5G^TCGAC3
 +
|
|US$56
|US$56
|2,000U @ 20,000U/ml
|2,000U @ 20,000U/ml
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!XbaI
!XbaI
|5T^CTAGA3
|5T^CTAGA3
 +
|
|US$63
|US$63
|3,000U @ 20,000U/ml
|3,000U @ 20,000U/ml
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|YES
|YES
|}
|}
 +
 +
====Double Digest Buffers====
 +
 +
 +
====Lab Location====
====Lab Location====
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====Store====
====Store====
-
*Available from Bio21 store? Yes  
+
*Available from Bio21 store? Yes
 +
*location: [Media:melbourne Erefrigerator |E-refrigerator] Needs account card. (see [[Media:melbourne daisy |Daisy]]).
-
====Supplier:====
 
-
*Supplier:
 
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*Suppliers part no:
 
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*Contact details for supplier:
 
-
*Email:
 
-
*Phone:
 
====Manufacturer:====
====Manufacturer:====

Revision as of 06:52, 11 July 2007

<return to list of primary reagents> <team home page>

Contents

Physical data

  • Description: NEB Restriction enzymes are used generally in the Lab.
NEB restriction enzymes
Enzyme Cut site 100% buffers Cost No of U Part no/data location In store
PstI 5CTGCA^G3 US$58 10,000 @ 20,000U/ml [http://www.neb.com/nebecomm/products/productR0140.asp NEB.R0140S] YES
EcoRI 5G^AATTC3 US$53 10,000 @ 20,000U/ml [http://www.neb.com/nebecomm/products/productR0101.asp NEB.R0101S] YES
HaeII 5RGCGC^Y3 US$53 2,000U @ 20,000U/ml [http://www.neb.com/nebecomm/products/productR0107.asp NEB.R0107S] YES
SalI 5G^TCGAC3 US$56 2,000U @ 20,000U/ml [http://www.neb.com/nebecomm/products/productR0138.asp NEB.R0138S] YES
XbaI 5T^CTAGA3 US$63 3,000U @ 20,000U/ml [http://www.neb.com/nebecomm/products/productR0145.asp NEB.R0145S] YES

Double Digest Buffers

Lab Location

  • Location see table
  • Permision to use required? No


Store

  • Available from Bio21 store? Yes
  • location: [Media:melbourne Erefrigerator |E-refrigerator] Needs account card. (see Daisy).


Manufacturer:




  • PhillipDodson 03:48, 1 July 2007 (EDT):Primary Reagent Template 1/July/2007