Melbourne/meeting 3 August 2007

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Contents

5:30 Action points from last meeting:

  • Alisa& Pat: Wiki "Parts_used status" up to date? -(outstanfding from 19/7/07)
  • Jan: Lecture Timetables spread sheet to be emailed to all
  • Pat & Alisa: Plan for red light receptor on wiki
  • Alisa, Paul & Jan: Review blue light theory
  • Jan:Theory for blue light on wiki
  • Jan:ComP fusion to be ordered.
  • Jan:Production of sequence for blue light receptor? " -designed
  • Jan:Plan for blue light on wiki by 17th August
  • Jan:<b/>Order red and blue filters.
  • <b>Paul: Status of #BBa-M30109 & #BBa-V1012 lost in transit?
  • Jan: Production of sequence for blue light receptor? " -designed
  • Craig: Alkaline lysis technique for miniprep to be documented
  • Cheng: Daisy to produce competent cells of DB3.1 and Enz- strains.

5:45 Status Reports: (5 minutes each including any discussion)

  • Alisa: Ligation experiments progress.
    • Problem: 1.2KB genes between biobrick forward & reverse primers not being visualised in colony PCR with 1.5min extn time. (300bp fragments do amplify)
      • - craig same ligation worked fine [no BSA, no gel purification, no Alkaline phosphatase used]
      • - craig very high 10mg/ml DNA yield from miniprep using alkaline lysis technique.
      • - Alisa: doesn't believe it is worth investigating any further except as experiments are done.
  • Pat & Alisa: Red light receptor progress/design/plan- needs?
    • phase 1 osmolarity test of YFP under enz
    • phase 2 Red light driving YFP
    • Phil would like to see lacZ coliphotography parts available for tests due to higher sensitivity of enzyme output.
  • Jan: Blue light receptor design/plan - needs?
    • Phil sugests using prism for optimal colour discovery before ordering filters, Projector as source.
    • Review to be cionducted with Jan, Paul & Alisa
    • No need to order any apperatus yet.
  • Phil: Gas vesicles progress/design/plan - needs?
    • Plan up to basic biobrick complete,
    • First mutagenesis in progress using complementry primers hand designed, and one overlapping and one computer generated using stratagene program.
    • Plan for support parts/test harness required.
    • Lac promotor and antibiotic death plasmids required.
  • Kasia: Documentation progress vs last report/ new problems/ needs.-No report

6.15 New Agenda Items:

  • Phil: Individuals desired time commitment:
  • Alisa: To Invite Chris to join team as full member.
  • Phil: Administrative Timetable: (11 Weeks to go)
  • Next meeting: <b/> 10th August (Friday) 5.30PM agreed but latter changed to ) 5:15PM.

7:30 End

Igem Administrative Timetable:

1 September Final team rosters due.
1 September Jamboree attendance fees due $100USD per team

member.

26 October Project and part documentation due DNA received USA.
26 October BioBrick Part DNA received by the Registry.
3-4 November iGEM Competition Jamboree, MIT, USA.

.


Documentation wiki status:

    • Priority should be on plans and experimental report analysis and conclusions-----Alisa .
    • If only Pat & Alisa doing lab work not much documentation needed.
    • Equipement:
      • MissingLas3000 method (in lab notebook)
      • Missing DNA measurement protocol (eppendorf)
    • Reagents pages incomplete:
      • Some location pictures not finished or uploaded.
      • Some newer reagents not entered.
    • Secondary reagents pages not made:
      • LB, Plates, TAE , NZY+ Broth
    • Protocol part:
      • No connections from Protocols to reagents.
      • Ligation protocol missing
    • Lab notebook:
      • Format uses too many lines to see connections between days.
      • Latter part missing links
      • Can table of contents be restricted to date level?
    • Experimental reports:
      • Lacks discussion/conclusion
      • Ligation experiment missing
      • Lattest few gells not marked up and entered.
    • Project overview link broken
    • Background:
      • GFP missing ,
      • Red photo sensor to GFP test harness missing.
    • Plan: Only Gas vesicles plan entered.
IndividualDesired time committment
Pat About 4h/wk Monday afternoon or Tuesday morning
Alisa About 4h/wk - Thursday afternoon
Jan About 10h/pw - flexible weekday mornings
Phil About 18h/pw - Thursday morning, Friday, Saturday or Sunday,