Melbourne/meeting 3 August 2007
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Revision as of 02:05, 10 August 2007 by PhillipDodson (Talk | contribs)
Contents |
5:30 Action points from last meeting:
- Alisa& Pat: Wiki "Parts_used status" up to date? -(outstanfding from 19/7/07)
- Jan: Lecture Timetables spread sheet to be emailed to all
- Pat & Alisa: Plan for red light receptor on wiki
- Alisa, Paul & Jan: Review blue light theory
- Jan:Theory for blue light on wiki
- Jan:ComP fusion to be ordered.
- Jan:Production of sequence for blue light receptor? " -designed
- Jan:Plan for blue light on wiki by 17th August
- Jan:<b/>Order red and blue filters.
- <b>Paul: Status of #BBa-M30109 & #BBa-V1012 lost in transit?
- Jan: Production of sequence for blue light receptor? " -designed
- Craig: Alkaline lysis technique for miniprep to be documented
- Cheng: Daisy to produce competent cells of DB3.1 and Enz- strains.
5:45 Status Reports: (5 minutes each including any discussion)
- Alisa: Ligation experiments progress.
- Problem: 1.2KB genes between biobrick forward & reverse primers not being visualised in colony PCR with 1.5min extn time. (300bp fragments do amplify)
- - craig same ligation worked fine [no BSA, no gel purification, no Alkaline phosphatase used]
- - craig very high 10mg/ml DNA yield from miniprep using alkaline lysis technique.
- - Alisa: doesn't believe it is worth investigating any further except as experiments are done.
- Problem: 1.2KB genes between biobrick forward & reverse primers not being visualised in colony PCR with 1.5min extn time. (300bp fragments do amplify)
- Pat & Alisa: Red light receptor progress/design/plan- needs?
- phase 1 osmolarity test of YFP under enz
- phase 2 Red light driving YFP
- Phil would like to see lacZ coliphotography parts available for tests due to higher sensitivity of enzyme output.
- Jan: Blue light receptor design/plan - needs?
- Phil sugests using prism for optimal colour discovery before ordering filters, Projector as source.
- Review to be cionducted with Jan, Paul & Alisa
- No need to order any apperatus yet.
- Phil: Gas vesicles progress/design/plan - needs?
- Plan up to basic biobrick complete,
- First mutagenesis in progress using complementry primers hand designed, and one overlapping and one computer generated using stratagene program.
- Plan for support parts/test harness required.
- Lac promotor and antibiotic death plasmids required.
- Kasia: Documentation progress vs last report/ new problems/ needs.-No report
6.15 New Agenda Items:
- Phil: Individuals desired time commitment:
- Alisa: To Invite Chris to join team as full member.
- Phil: Administrative Timetable: (11 Weeks to go)
- Next meeting: <b/> 10th August (Friday) 5.30PM agreed but latter changed to ) 5:15PM.
7:30 End
Igem Administrative Timetable:
1 September | Final team rosters due. |
1 September | Jamboree attendance fees due $100USD per team
member. |
26 October | Project and part documentation due DNA received USA. |
26 October | BioBrick Part DNA received by the Registry. |
3-4 November | iGEM Competition Jamboree, MIT, USA. |
.
Documentation wiki status:
- Priority should be on plans and experimental report analysis and conclusions-----Alisa .
- If only Pat & Alisa doing lab work not much documentation needed.
- Equipement:
- MissingLas3000 method (in lab notebook)
- Missing DNA measurement protocol (eppendorf)
- Reagents pages incomplete:
- Some location pictures not finished or uploaded.
- Some newer reagents not entered.
- Secondary reagents pages not made:
- LB, Plates, TAE , NZY+ Broth
- Protocol part:
- No connections from Protocols to reagents.
- Ligation protocol missing
- Lab notebook:
- Format uses too many lines to see connections between days.
- Latter part missing links
- Can table of contents be restricted to date level?
- Experimental reports:
- Lacks discussion/conclusion
- Ligation experiment missing
- Lattest few gells not marked up and entered.
- Project overview link broken
- Background:
- GFP missing ,
- Red photo sensor to GFP test harness missing.
- Plan: Only Gas vesicles plan entered.
Individual | Desired time committment |
---|---|
Pat | About 4h/wk Monday afternoon or Tuesday morning |
Alisa | About 4h/wk - Thursday afternoon |
Jan | About 10h/pw - flexible weekday mornings |
Phil | About 18h/pw - Thursday morning, Friday, Saturday or Sunday, |