BerkiGEM2007-DavidConstructionFile102

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Revision as of 06:34, 5 June 2007 by Dtulga (Talk | contribs)
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Biobrickificreation of pir I716102 (R6K Origin Inducer)
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PCR pir-F/pir-IR on pir116		(375 bp, gp = A)
PCR pir-IF/pir-R on pir116		(599 bp, gp = B)
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PCR pir-F/pir-R on A+B			(949 bp, BglII/XhoI)
Sub into pBca9145-Bca1144		(BglII/XhoI, 2063+910, L)
Product is pBca9145-I716102-pir
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dt001-pir-F   Forward Cloning of pir   ccaaaAGATCTatgagactcaaggtcatgatg
dt002-pir-IF  Forward Removing the BglII site in pir  gaagagttagaCcttaacattattg
dt003-pir-IR  Reverse Removing the BglII site in pir  caataatgttaagGtctaactcttc
dt004-pir-R   Reverse Cloning of pir   gctagCTCGAGttaGGATCCtcaccccttagcttttttgg