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Contents

What We've Accomplished

Week's (Ambitious) Goals

Wednesday 5/30

  1. Get all protocols
  2. Identify materials/prepare order
  3. Design Primers
  4. Learn about budget/POs

Thursday 5/31

  1. Do primer order
  2. Start conjugation practice
  3. Confirm restriction enzymes, ligases
  4. Order confirmed/needed materials
  5. Team Revew Meeting
  6. Draft Thank-You Letters for our Sponsors

Friday 6/1

  1. Evaluate/continue conjugation, practice electroporation for E. coli
  2. Revise proposal to include possibility of screening with alginate beads and fluorocytometer
  3. Meeting with Tim: Budgets/protocols, Pfizer/fundraising, iGEM registration, beads

Materials We Need

Primers: Need to Buy

Error-Prone PCR: Need to Buy

Plasmids: Need to Buy?

Restriction Enzymes: Need to Buy?

Ligases: Need to Buy?

Short-Term To-Do List

Lab Orientation: COMPLETED!

Lab Safety Training: Not Completed (Dave, Rahul, and Christian are scheduled for a training session on 5/30/07. Danny will be taking it next week)

Design of Primers: Not Completed (Dave, please send me info about the finished primers when ready)

Ordering of Primers: Not Completed

Gathering of Protocols: Not Completed (Chris, please send me the protocols when they are gathered)

Ordering of Error-Prone PCR Materials: Not completed

Thank-You Letters sent to Pfizer: COMPLETED!

Thank-You Letters sent to BU ppl: Not completed

Relevant Publications and Links

http://www.shewybase.bu.edu

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