Caltech/Project

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==<center>Project Background</center>==
 
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[[Caltech/Project/phage|Introduction to Bacteriophage λ]]
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==<center>Project Overview</center>==
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Briefly, our project relies on controlling key viral developmental processes in a target-cell specific manner. In our design, the engineered viruses are capable of entering all cells. The viruses are engineered to lack the native copy of a key developmental gene, while containing a second, regulated, copy which is only expressed when the virus infects specific target cells. Thus, viruses infecting non-target cells stall early in their development and are quickly destroyed by the host. Viruses infecting target cells, however, manage to express these essential genes and successfully complete their infection cycle.
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==<center>Project Details</center>==
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As an initial mechanism to target viruses to specific cell types, we will place the viral developmental genes under riboregulator control. Viral mRNAs for the regulated developmental genes will express with a stem loop sequestering ribosome binding sites, preventing translation. Specific mRNA in target E. coli will invade the stem loop, freeing the ribosome binding site and allowing proper translation. We believe this approach is more general than methods which might target specific cell-surface markers. Furthermore, if this method works, it would be possible in principle to extend viral mRNA regulation using aptamers capable of recognizing subtle signals such as post-translational modification.
 +
We selected the viral developmental genes <i>N, Q,</i> and <i>cro</i> as promising targets for regulation. <i>N</i> and <i>Q</i> are antiterminators required for λ to transcribe its full set of genes. Viruses lacking these genes stall at extremely early developmental stages and are completely inviable, barely producing any viral mRNA. <i>cro</i> biases the virus' decision to either lyse a target cell or integrate into the host's DNA, possibly allowing us to selectively 'rewire' host bacteria.
 +
 +
Choosing an appropriate λ strain poses a challenge. Existing strains with defective <i>N, Q,</i> and <i>cro</i> genes lack unique restriction sites to clone our constructs into. Therefore, we will utilize recombineering to introduce introduce these mutations into phages specifically designed to accept cloning inserts.
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[[Image:Caltech_2007_overview.jpg|center|frame|An overview of the Caltech 2007 iGEM project. We knock out the native copy of a key developmental gene, then add in a second copy under riboregulated control. Infection will only proceed in cells with the correct trans-activating RNA 'key'.]]
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==<center>Project Details</center>==
The project consisted of five individual parts, each assigned to one team member, and categorized into one of three independent principles underlying the project:
The project consisted of five individual parts, each assigned to one team member, and categorized into one of three independent principles underlying the project:
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* [[Caltech/Project/Recombineering|Recombineering]] the cro, N, and Q amber mutation genes into lambda zap
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* [[Caltech/Project/Recombineering|Recombineering]] amber mutations into the N, Q, and cro genes of λ-Zap
* [[Caltech/Project/Riboregulator|Riboregulator]] design and testing
* [[Caltech/Project/Riboregulator|Riboregulator]] design and testing
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*Titering [[Caltech/Project/Cro|Cro]], [[Caltech/Project/N|N-antiterminator]], and [[Caltech/Project/Q|Q-antiterminator]] construct-containing bacterial to test construct-mediated rescue of lytic activity (in amber phage)
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* Expressing heterologous [[Caltech/Project/Cro|Cro]], [[Caltech/Project/N|N-antiterminator]], and [[Caltech/Project/Q|Q-antiterminator]] in ''E. coli'' to test for complementation of phage with amber mutations in the native copy of each gene
==<center>Current Status</center>==
==<center>Current Status</center>==
-
As the recombineering, testing of riboregulators, and titering processes will take place concurrently, we needed to find a simpler way to regulate viral protein concentrations in the cells. To this end, ''E. coli'' strains have been constructed that contain a low-copy plasmid construct where one of three key developmental viral genes - coding for the cro, N, or Q proteins - is regulated by a tetracycline-dependent promoter. A constitutive promoter (J23100, the stronger promoter, or J23116, the weaker one) produces a steady stream of tetracycline repressor (tetR), which substitutes for the cis repressor in repressing protein levels. The addition of anhydrotetracycline (aTc, acting as the trans activator) inactivates the tetracycline repressor and leads to the production of the respective viral protein in the ''E. coli'' cells. This allows us to control the concentration of viral protein produced in the cells by adding varying amounts of aTc to the bacterial growth media.
+
As the recombineering, testing of riboregulators, and titering processes took place concurrently, we needed to find a simpler way to regulate viral protein concentrations in the cells. To this end, ''E. coli'' strains have been constructed that contain a low-copy plasmid construct where one of three key developmental viral genes - coding for the cro, N, or Q proteins - is regulated by a tetracycline-dependent promoter. A constitutive promoter produces a steady stream of tetracycline repressor (tetR), which substitutes for the cis repressor in repressing protein levels. The addition of anhydrotetracycline (aTc, acting as the trans activator) inactivates the tetracycline repressor and leads to the production of the respective viral protein in the ''E. coli'' cells. This allows us to control the concentration of viral protein produced in the cells by adding varying amounts of aTc to the bacterial growth media.
* [[Caltech/Project/Constructs|Explanation of Construct]]
* [[Caltech/Project/Constructs|Explanation of Construct]]
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* [[Caltech/Project/Construct List|List of Cloned Constructs]]
 
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Titering experiments where cro, N, and Q amber phages were allowed to infect D1210 cells containing the built construct show that heterologous N and Q can complement phages with amber mutations in the respective genes. Adding a cis-repressor to the Q construct lowered production of Q even further, as it eliminated lysis completely. We were unable to express sufficient cro from a plasmid to rescue lytic behavior of the amber cro mutant phage.
Titering experiments where cro, N, and Q amber phages were allowed to infect D1210 cells containing the built construct show that heterologous N and Q can complement phages with amber mutations in the respective genes. Adding a cis-repressor to the Q construct lowered production of Q even further, as it eliminated lysis completely. We were unable to express sufficient cro from a plasmid to rescue lytic behavior of the amber cro mutant phage.
* [[Caltech/Project/Titering Results|Summary of Titering Results]]
* [[Caltech/Project/Titering Results|Summary of Titering Results]]
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Multiple riboregulator designs are being tested (for both activation and repression levels), and successful designs will be cloned into the plasmid constructs. So far, cis construct number 3 and its accompanying trans combinations (cis3/trans1 and cis3/trans2) seem the most promising. Phages resulting from the recombineering process are also being screened for successful N and Q amber mutants.
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Multiple riboregulator designs are being tested (for both activation and repression levels), and successful designs will be cloned into the plasmid constructs. So far, cis construct number 3 and its accompanying trans combinations (cis3trans1 and cis3trans2) seem the most promising. Phages resulting from the recombineering process are also being screened for successful N and Q amber mutants.
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* [[Caltech/Project/Riboregulator Results|''Trans'' Activation Data]]
* [[Caltech/Project/Riboregulator Results|''Trans'' Activation Data]]
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==<center>Future Work</center>==
==<center>Future Work</center>==
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We are currently testing the ability of our best cis/trans combinations (cis3/trans1 and cis3/trans2) to regulate Q expression levels. We will titer the Q amber mutant phage on cells containing the cis3-repressed Q, either with or without the appropriate trans-activating RNA. A positive result (no plaques on cis containing strains, plaques on cis-trans strains) would show successful integration of two independent project components (N/Q protein dependent lysis switch, and the “lock and key” riboregulator). Successful integration demonstrates that standardization on multiple levels (BioBrick parts making each construct, and the more abstract merging of riboregulators into viral decision-making) can allow rapid construction of complex synthetic biological control pathways.
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The future of the project lies in confirming trans activation: that is, to prove protein concentration is several times greater in aTc-saturated trans-containing cells as compared to aTc-saturated strains with no trans plasmid. The successful complementary cis-trans pairs can then be incorporated into the N-J23100 and Q-J23116 constructs. Cis repression in the N-construct has yet to be tested, but Q’s results would imply successful repression of lytic action. Based on the presence of plaques on D1210-N and D1210-Q strains even without aTc, even a small amount of trans-activation woudl allow amber phages to successfully enter the lytic cycle.  
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Looking farther forward, we have several tasks to finish. First, we have several more cis/trans combinations to test by flow cytometry. Next, we need to check whether cis repression in the N construct is sufficient to inhibit complementation of N amber mutant phage. As with the cis3-Q/trans1 and cis3-Q/trans2 combinations, we will have to test promising cis/trans combinations with both N and Q.
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Cis repression of the N and Q constructs can be tested by further titering.  A quantitative measure of the increase in protein concentration upon trans-activation will be obtained by fluorescence measurements by flow cytometry. So far, trans activation has been shown in YFP Quanta experiments (cis3-trans1 and cis3-trans2 seem the most promising), although combinations remain that need to be tested. The ultimate test, though, lies in titering amber mutant phage into strains containing both cis and trans plasmids. A positive result (no plaques on cis containing strains, plaques on cis-trans strains) would show successful integration of two independent project components (N/Q protein dependent lysis switch, and the “lock and key” riboregulator). Successful integration demonstrates that standardization on multiple levels (BioBrick parts making each construct, and the more abstract merging of riboregulators into viral decision-making) can allow rapid construction of complex synthetic biological control pathways.
+
The final step, then, would be to finish recombineering amber mutations into the N or Q gene of λ-Zap. We will then clone our cis-repressed N and Q constructs into the multiple cloning site of the mutant λ-Zap. These phage will be used to infect ''E. coli'' with or without the appropriate trans plasmid. If our riboregulation is sufficient, the phage would only lyse those cells containing the trans plasmid.
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Our current top priority is now to transform the trans1 and trans2 plasmids into the cis3-Q construct cell, titer and check for the presence of plaques. Once successful, we can clone the construct entirely into the recombineered amber phage. In the final system, infecting amber λ-Zap virus will selectively lyse those cells that express the specific trans RNA in their transcriptional profile.
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==Applications==
 +
We see several applications for this research. Most immediately, we believe that selective bacteriophage could be used for containment in the event of an accidental environmental release of engineered bacteria. The phage could specifically target the released bacteria, without harming other wild species of bacteria.
 +
Next, the phage could be used in a laboratory setting to help prevent the formation of 'cheaters' in an engineered  population. If a genetic construct places host cells at a growth disadvantage, and cells which can eliminate the construct would quickly take over the population. Our phage could be targeted such that they only kill cells which no longer display their desired function, allowing for more direct control over cheating.
 +
Finally, the lessons learned using our system may be applicable to medical applications involving gene therapy. In such an application, we would aim to target specific cells. These could allow tissue-specific treatment, or specific targeting of cancerous cells.
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Latest revision as of 03:54, 27 October 2007


Caltech phage.jpg

iGEM 2007

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Project Overview

Briefly, our project relies on controlling key viral developmental processes in a target-cell specific manner. In our design, the engineered viruses are capable of entering all cells. The viruses are engineered to lack the native copy of a key developmental gene, while containing a second, regulated, copy which is only expressed when the virus infects specific target cells. Thus, viruses infecting non-target cells stall early in their development and are quickly destroyed by the host. Viruses infecting target cells, however, manage to express these essential genes and successfully complete their infection cycle.

As an initial mechanism to target viruses to specific cell types, we will place the viral developmental genes under riboregulator control. Viral mRNAs for the regulated developmental genes will express with a stem loop sequestering ribosome binding sites, preventing translation. Specific mRNA in target E. coli will invade the stem loop, freeing the ribosome binding site and allowing proper translation. We believe this approach is more general than methods which might target specific cell-surface markers. Furthermore, if this method works, it would be possible in principle to extend viral mRNA regulation using aptamers capable of recognizing subtle signals such as post-translational modification.

We selected the viral developmental genes N, Q, and cro as promising targets for regulation. N and Q are antiterminators required for λ to transcribe its full set of genes. Viruses lacking these genes stall at extremely early developmental stages and are completely inviable, barely producing any viral mRNA. cro biases the virus' decision to either lyse a target cell or integrate into the host's DNA, possibly allowing us to selectively 'rewire' host bacteria.

Choosing an appropriate λ strain poses a challenge. Existing strains with defective N, Q, and cro genes lack unique restriction sites to clone our constructs into. Therefore, we will utilize recombineering to introduce introduce these mutations into phages specifically designed to accept cloning inserts.

An overview of the Caltech 2007 iGEM project. We knock out the native copy of a key developmental gene, then add in a second copy under riboregulated control. Infection will only proceed in cells with the correct trans-activating RNA 'key'.


Project Details

The project consisted of five individual parts, each assigned to one team member, and categorized into one of three independent principles underlying the project:

Current Status

As the recombineering, testing of riboregulators, and titering processes took place concurrently, we needed to find a simpler way to regulate viral protein concentrations in the cells. To this end, E. coli strains have been constructed that contain a low-copy plasmid construct where one of three key developmental viral genes - coding for the cro, N, or Q proteins - is regulated by a tetracycline-dependent promoter. A constitutive promoter produces a steady stream of tetracycline repressor (tetR), which substitutes for the cis repressor in repressing protein levels. The addition of anhydrotetracycline (aTc, acting as the trans activator) inactivates the tetracycline repressor and leads to the production of the respective viral protein in the E. coli cells. This allows us to control the concentration of viral protein produced in the cells by adding varying amounts of aTc to the bacterial growth media.

Titering experiments where cro, N, and Q amber phages were allowed to infect D1210 cells containing the built construct show that heterologous N and Q can complement phages with amber mutations in the respective genes. Adding a cis-repressor to the Q construct lowered production of Q even further, as it eliminated lysis completely. We were unable to express sufficient cro from a plasmid to rescue lytic behavior of the amber cro mutant phage.

Multiple riboregulator designs are being tested (for both activation and repression levels), and successful designs will be cloned into the plasmid constructs. So far, cis construct number 3 and its accompanying trans combinations (cis3/trans1 and cis3/trans2) seem the most promising. Phages resulting from the recombineering process are also being screened for successful N and Q amber mutants.

Future Work

We are currently testing the ability of our best cis/trans combinations (cis3/trans1 and cis3/trans2) to regulate Q expression levels. We will titer the Q amber mutant phage on cells containing the cis3-repressed Q, either with or without the appropriate trans-activating RNA. A positive result (no plaques on cis containing strains, plaques on cis-trans strains) would show successful integration of two independent project components (N/Q protein dependent lysis switch, and the “lock and key” riboregulator). Successful integration demonstrates that standardization on multiple levels (BioBrick parts making each construct, and the more abstract merging of riboregulators into viral decision-making) can allow rapid construction of complex synthetic biological control pathways.

Looking farther forward, we have several tasks to finish. First, we have several more cis/trans combinations to test by flow cytometry. Next, we need to check whether cis repression in the N construct is sufficient to inhibit complementation of N amber mutant phage. As with the cis3-Q/trans1 and cis3-Q/trans2 combinations, we will have to test promising cis/trans combinations with both N and Q.

The final step, then, would be to finish recombineering amber mutations into the N or Q gene of λ-Zap. We will then clone our cis-repressed N and Q constructs into the multiple cloning site of the mutant λ-Zap. These phage will be used to infect E. coli with or without the appropriate trans plasmid. If our riboregulation is sufficient, the phage would only lyse those cells containing the trans plasmid.

Applications

We see several applications for this research. Most immediately, we believe that selective bacteriophage could be used for containment in the event of an accidental environmental release of engineered bacteria. The phage could specifically target the released bacteria, without harming other wild species of bacteria.

Next, the phage could be used in a laboratory setting to help prevent the formation of 'cheaters' in an engineered population. If a genetic construct places host cells at a growth disadvantage, and cells which can eliminate the construct would quickly take over the population. Our phage could be targeted such that they only kill cells which no longer display their desired function, allowing for more direct control over cheating.

Finally, the lessons learned using our system may be applicable to medical applications involving gene therapy. In such an application, we would aim to target specific cells. These could allow tissue-specific treatment, or specific targeting of cancerous cells.