Chiba/Lab Notebook

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Lux TEAM

FliC TEAM

  • LINKER LIGATION
    • pUC19-FliC(vector)の制作
  1. Inverse PCR（五種類のF-Primer,R-Primer→15通りのPCR）
    1. Check Size
    2. Digestion(EcoRI,NcoI)
    3. Zymo Clean(40μ溶出)→SAP（50μ系）→Zymo Clean（30μ溶出）


  1. lasmid check
    1. Digestion Test
    2. Sequence
  2. Expression Test of a FliC-Histag
    1. SDS-PAGE
    2. Western-Blotting
    3. Magnetic beads
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