Chiba/Lab Notebook

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Lux TEAM

FliC TEAM

  • LINKER LIGATION
    • pUC19-FliC(vector)の制作
  1. pUC19-FliCをInverse PCR（五種類のF-Primer,R-Primer→15通りのPCR）
    1. Check Size
    2. Digestion(EcoRI,NcoI)
    3. Zymo Clean(40μ溶出)→SAP（50μ系）→Zymo Clean（30μ溶出）
  2. Histag,Histag-antiをアニール
    1. Digestion(EcoRI,NcoI)
  3. Ligation
  4. Transformation
  5. miniprep
  6. Digestion test
  7. Sequence→FliCに6×His配列が挿入されていることを確認
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