Chiba/Weekly
From 2007.igem.org
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== Week4(9/9~9/15) == | == Week4(9/9~9/15) == | ||
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== Week5(9/16~9/22) == | == Week5(9/16~9/22) == | ||
Revision as of 20:14, 10 September 2007
Contents |
Week1(8/16~8/23)
Monday,August,17,2007
- pUC-FliCのPCR
| F/R | FliC-207 | FliC-228 | FliC-240 | FliC-252 | FliC-267 |
|---|---|---|---|---|---|
| FliC-207 | ① | ||||
| FliC-228 | ② | ③ | |||
| FliC-240 | ④ | ⑤ | ⑥ | ||
| FliC-252 | ⑦ | ⑧ | ⑨ | ⑩ | |
| FliC-267 | ⑪ | ⑫ | ⑬ | ⑭ | ⑮ |
| Recipe(単位：μ) | ① | ② | ③ | ④ | ⑤ | ⑥ | ⑦ | ⑧ | ⑨ | ⑩ | ⑪ | ⑫ | ⑬ | ⑭ | ⑮ |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| pUC19-FliC | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
| FliC-207F | 5 | ||||||||||||||
| FliC-207R | 5 | 5 | 5 | 5 | 5 | ||||||||||
| FliC-228F | 5 | 5 | |||||||||||||
| FliC-228R | 5 | 5 | 5 | 5 | |||||||||||
| FliC-240F | 5 | 5 | 5 | ||||||||||||
| FliC-240R | 5 | 5 | 5 | ||||||||||||
| FliC-252F | 5 | 5 | 5 | 5 | |||||||||||
| FliC-252R | 5 | 5 | |||||||||||||
| FliC-267F | 5 | 5 | 5 | 5 | 5 | ||||||||||
| FliC-267R | 5 | ||||||||||||||
| 10×Buffer | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 |
| dNTPmix | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 |
| Vent | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
| dH2O | 68 | 68 | 68 | 68 | 68 | 68 | 68 | 68 | 68 | 68 | 68 | 68 | 68 | 68 | 68 |
| Total | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 |
Week2(8/24~8/31)
Week3(9/1~9/8)
Week4(9/9~9/15)
