Edinburgh/Yoghurt/Design

From 2007.igem.org

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[[Edinburgh]] > '''Yoghurt'''
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[[Edinburgh/Yoghurt| Introduction]] | [[Edinburgh/Yoghurt/Applications|Applications]] | [[Edinburgh/Yoghurt/Objectives|Objectives]] | [[Edinburgh/Yoghurt/Design|Design]] | [[Edinburgh/Yoghurt/Modelling|Modelling]] | [[Edinburgh/Yoghurt/Wet Lab|Wet Lab]] | [[Edinburgh/Yoghurt/References|References]]
[[Edinburgh/Yoghurt| Introduction]] | [[Edinburgh/Yoghurt/Applications|Applications]] | [[Edinburgh/Yoghurt/Objectives|Objectives]] | [[Edinburgh/Yoghurt/Design|Design]] | [[Edinburgh/Yoghurt/Modelling|Modelling]] | [[Edinburgh/Yoghurt/Wet Lab|Wet Lab]] | [[Edinburgh/Yoghurt/References|References]]
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=Design=
 
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==Vanillin Project==
 
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One of our aims for iGEM is to construct a synthetic pathway to produce vanillin from the amino acid tyrosine.  There are five genes in this pathway, which are present in three different organisms.
 
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====Vanillin Biosynthesis Pathway====
 
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[[Image:Vanillin synthesis pathway.JPG]]
 
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The ''Sam5'' and ''Sam8'' genes are from the microorganism ''Saccharothrix espanaensis''.  ''Sam8'' encodes a tyrosine ammonia lyase enzyme, and ''Sam5'' a hydroxylse enzyme.  These genes are being kindly doanted by Anton Linnenbrink from the Institute of Pharmaceutical Sciences in Germany.
 
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The caffeic acid-3-0-methyltransferase gene is part of the lignin biosynthesis pathway present in plants.  Our initial plan is to extranct the gene out of Alfalfa using a PCR based method.  However if this method does not work we may attempt to purify ''COMT'' cDNA or have the gene artifically synthesised by GeneArt.
 
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Both ''fcs'' and ''ech'' genes are present in a variety of microorganisms.  After much searching we have identified ech and fcs genes in ''Pseudomonas fluorescens'', which have a minimal number of forbidden restriction sites.  The PstI site within ech is located at the 3' terminal and shall be mutated out during the PCR amplification of the gene from ''P. fluorescens''.
 
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[[Edinburgh/Yoghurt| Introduction]] | [[Edinburgh/Yoghurt/Applications|Applications]] | [[Edinburgh/Yoghurt/Objectives|Objectives]] | [[Edinburgh/Yoghurt/Design|Design]] | [[Edinburgh/Yoghurt/Modelling|Modelling]] | [[Edinburgh/Yoghurt/Wet Lab|Wet Lab]] | [[Edinburgh/Yoghurt/References|References]]
[[Edinburgh/Yoghurt| Introduction]] | [[Edinburgh/Yoghurt/Applications|Applications]] | [[Edinburgh/Yoghurt/Objectives|Objectives]] | [[Edinburgh/Yoghurt/Design|Design]] | [[Edinburgh/Yoghurt/Modelling|Modelling]] | [[Edinburgh/Yoghurt/Wet Lab|Wet Lab]] | [[Edinburgh/Yoghurt/References|References]]
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Revision as of 13:14, 23 September 2007

Introduction | Applications | Objectives | Design | Modelling | Wet Lab | References



Introduction | Applications | Objectives | Design | Modelling | Wet Lab | References

Retrieved from "http://2007.igem.org/wiki/index.php/Edinburgh/Yoghurt/Design"