Glasgow

From 2007.igem.org

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[http://www.gla.ac.uk https://static.igem.org/mediawiki/2007/5/5b/Glasgow_header1.png]
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|[https://2007.igem.org/Glasgow/Plan <font face=georgia color=##3366CC size=5><b>Project</b></font>]
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|[https://2007.igem.org/Glasgow/Meet_the_team <font face=georgia color=#3366CC size=5><b>Meet The Team</b></font>]
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|+<span style="color:blue">'''What would you like to know more about?'''</span>
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|[https://2007.igem.org/Glasgow/Wetlab <font face=georgia color=##3366CC size=5><b>Wetlab</b></font>]
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|[https://2007.igem.org/Glasgow/Modeling <font face=georgia color=#3366CC size=5><b>Modelling</b></font>]
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|<center>[[Image:Drylab.png]]<br> [[Image:WetlabLog.png]]</center>
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<center>[[Image: Glasgow_group_picture.jpg|800px]]</center>
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== A wee bit about our project....==
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Large amounts of pollutants enter the environment each year.  Biosensors are often used for the detection of environmentally relevant pollutants in both groundwater and soil. Our current work involves the use of new Biobricks that we have designed, in conjunction with existing Biobricks in order to design and create two biosensor systems that detect xylene and salicylate respectively.
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In these systems, the pollutant forms a complex with a constitutively expressed transcriptional regulator, which in turn drives the expression of proteins allowing the bacteria to metabolize or degrade the pollutant. By replacing the genes that encode these proteins with a reporter gene an easily detected signal, such as pH or colour change is produced, thus creating a biosensor.
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Our project aimed to design and construct a completely novel type of self-powering electrochemical biosensor, called '''''ElectrEcoBlu'''''.  The novelty lies in the fact that the output signal is an electrochemical mediator which enables electrical current to be generated in a microbial fuel cell. '''''ElectrEcoBlu'''''  functions as a biosensor for a range of important and widespread environmental organic pollutants which stimulate the biosensor to produce its own electrical power output. The system has the potential to be used for self-powered long term ''in situ''  and online monitoring with an electrical readout.  Our approach exploited a range of state-of-the art modelling techniques to support the design and construction of this novel synthetic biological system. This was facilitated by the entire team - biologists and modellers - working in an integrated laboratory environment.
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The modeling team provides feedback to biologists. ''In silico'' simulations tend to be more flexible and quicker to complete than ''in vitro'' experiments, this allows us to guide our project more efficiently. First a common framework is decided upon that represents the desired biological system. This framework can then be interpreted in a number of ways which allow us to determine substrate concentration changes over time with respect to acknowledged parameters and initial concentrations (these may be found in literature, obtained experimentally or arbitrarily chosen).
 
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Simple dynamic modeling will allow us to spot general qualitative and quantitative trends, stochastic representation will provide a more realistic simulation of the actual interaction on a molecular and cellular level, and various sensitivity tests will highlight how precise constraints on key components must be for a stable response. By analysing the system in this way, conclusions can be drawn to allow further evolution of the framework to optimise its performance according to predetermined specifications.
 
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== Links to our Sponsors ==
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[http://www.gla.ac.uk http://ultragrid.east.isi.edu/images/glasgow-logo.png]
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[http://www.scottish-enterprise.com/ http://www.conscia.co.uk/images/logo/se.gif]
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[http://www.merck.com/ http://www.v2020.org/library-media/images/Merck.jpg]
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[http://www.carnegie-trust.org http://www.headsup.org.uk/content/mediaassets/images/cukt_logo.gif]
[http://www.ethz.ch/index_EN http://www.photogrammetry.ethz.ch/logos/eth-logo-140.gif]
[http://www.ethz.ch/index_EN http://www.photogrammetry.ethz.ch/logos/eth-logo-140.gif]
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[http://www.anachem.co.uk http://www.pro-4-pro.com/media/company/anachem_logo.jpg]
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[[Image:Glasgow header.png]]
 

Latest revision as of 14:50, 26 October 2007

https://static.igem.org/mediawiki/2007/5/5b/Glasgow_header1.png

Project Meet The Team Wetlab Modelling


Glasgow group picture.jpg

Our project aimed to design and construct a completely novel type of self-powering electrochemical biosensor, called ElectrEcoBlu. The novelty lies in the fact that the output signal is an electrochemical mediator which enables electrical current to be generated in a microbial fuel cell. ElectrEcoBlu functions as a biosensor for a range of important and widespread environmental organic pollutants which stimulate the biosensor to produce its own electrical power output. The system has the potential to be used for self-powered long term in situ and online monitoring with an electrical readout. Our approach exploited a range of state-of-the art modelling techniques to support the design and construction of this novel synthetic biological system. This was facilitated by the entire team - biologists and modellers - working in an integrated laboratory environment.


https://static.igem.org/mediawiki/2007/thumb/9/9d/Merck.jpg/150px-Merck.jpg http://www.scottish-enterprise.com/selogolrg.gif http://www.headsup.org.uk/content/mediaassets/images/cukt_logo.gif http://www.photogrammetry.ethz.ch/logos/eth-logo-140.gif http://www.pro-4-pro.com/media/company/anachem_logo.jpg

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