June 29
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'''''For [D]''''' | '''''For [D]''''' | ||
| - | * The inductions were done in 50 mL Glu M9 in 250 mL flask with | + | * The inductions were done in 50 mL Glu M9 in 250 mL flask with 1 ng/mL conc of aTc and 1,0.1 uL/mL of inoculum. (1*50 ng=0.05 ug == 5 uL of 10 ug/mL aTc). |
| - | + | ||
| + | * pLluxR.Y.pR.C and K12z1 were inoculated in LB. | ||
| - | + | * The flask containing the required OD was selected and was stored at 4 degrees. | |
| - | + | * Seven dilutions for pL.LuxR.Y.pR.C with 50 old:50 new 2xM9 along with -ve control: K12z1 were made. | |
| - | |||
| + | '''Microscopy''' | ||
| + | * Open Loop samples [B] were imaged. | ||
| - | + | ==Analysis== | |
| - | + | ||
Latest revision as of 17:15, 29 June 2007
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Experiments
For [C]
- The inductions were done in 50 mL Glu M9 in 250 mL flask with 5 ng/mL conc of aTc and 1,0.1 uL/mL of the inoculum. (5*50 ng=0.25 ug == 25 uL of 10 ug/mL aTc)
- pLLuxR.Y.pR.C and K12z1 were inoculated in LB.
- Filter required OD flask, store at 4 degrees.
- Seven dilutions for pL.LuxR.Y.pR.C were made with 50 old:50 new 2xM9 along with -ve control: K12Z1.
For [D]
- The inductions were done in 50 mL Glu M9 in 250 mL flask with 1 ng/mL conc of aTc and 1,0.1 uL/mL of inoculum. (1*50 ng=0.05 ug == 5 uL of 10 ug/mL aTc).
- pLluxR.Y.pR.C and K12z1 were inoculated in LB.
- The flask containing the required OD was selected and was stored at 4 degrees.
- Seven dilutions for pL.LuxR.Y.pR.C with 50 old:50 new 2xM9 along with -ve control: K12z1 were made.
Microscopy
- Open Loop samples [B] were imaged.
