Lab Orientation Checklist

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iGEM training list

Space

  • Bench use - generally Frank and Kevin's, borrow others upon request
  • Desk space - use Ingalls

Glassware

  • clean and dirty dishes
  • autoclave tape, foil

Gels

  • Gel equipment: agarose, precast, acrylamide, E-gel
  • Gel buffers, dyes, wax paper, loading/mixing tricks, etc.
  • Gel doc system
  • Blue light system for cutting gels
  • darkroom

Chemicals

  • Inventory management system
  • Weighing materials, chemicals, inventory
  • Chemical hood - what mix there?

Consumables

  • miniprep kits
  • enzymes
  • sterile pipettes
  • Growth media, agar
  • Plates, tips, etc.
  • Agarose
  • chemicals

Safety

  • Waste locations
  • sharps, pipettes, razors, needles, biohazard
  • lab coats
  • eyewear
  • burners

Storage of materials

  • Non-volatile, room temp stuff
  • Acids and bases
  • Alcohols
  • Flamables, volatiles
  • Small fridges, big fridge, -20 freezer, -80 freezer (what goes where)
  • -80 Freezer organization
  • coldroom

Ordering

  • procedure
  • packing slips

Sequencing, oligos, enzymes

  • Agencourt
  • NEB, enzyme inventory
  • IDT

Equipment

  • manuals
  • Thermocyclers
  • incubators
  • centrifuges
  • autoclave
  • pH calibration
  • nanodrop
  • plate reader

Software

  • nanodrop
  • plate reader
  • clone manager
  • affy system
  • database

Core facilities

  • Room 623 - ice, gel doc, lyophilizer, extra incubator, coldroom
  • Flow cytometer
  • Microscopy
  • RT PCR