McGill/September

From 2007.igem.org

< McGill
Revision as of 04:14, 25 October 2007 by Avi (Talk | contribs)
September 2007
Sa Su M Tu W Th F
1 2 3 4 5 6 17
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30

</td></tr>

Contents

September 2007

September 4

Performed a screening digest of E0434 & I5611 after a midiprep. Results: Not so good, however, linear cuts showed that the DNA was present, so ligation will go ahead.

September 5

  • Transformed I5611 & E0434 again in Top 10 cells. Used a 2 min heat shock time, and plated on Amp/Kan (E0434) and Amp (I5611) plates.
  • Did a large scale digest I5611 & E0434. However, there might not have been enough BsrGI enzyme added, hence cuts were not clear and pronounced. Digest must be performed again.

September 7

  • Ligated linear I5611 & E0434. Left overnight at room temperature instead of at 37C in a water bath.
  • Then performed a screening digest of I5611 & E0434. This proved unsuccessful again.

September 11

  • Got excellent colonies for all ligation plates (used- Insert:Vector [1:1, 1:2, 1:3, 1:4, 1:5]x2). Each plate had ~25 colonies.
  • Seeded 12 colonies from each plate, in 1X LB with Amp/Kan resistance. Thus, seeded 60 colonies in total, the culture tubes were then left in the shaker/incubator at 5.30pm, to be kept overnight.