Melbourne/4 July 07 Digest
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- | [[ | + | [[Melbourne/Lab Notebook Weeks 1-4|<Return to lab notebook>]] [[Melbourne|<Back to team home page>]] |
====Dates: ==== | ====Dates: ==== | ||
*Method Documentation Comenced:1 July 2007 | *Method Documentation Comenced:1 July 2007 | ||
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====Aim: ==== | ====Aim: ==== | ||
- | * | + | *Prepare sufficient quantities of kit and other plasmids that are intended to be used in project. |
+ | *Confirm of contents of minipreped plasmids by digest. | ||
====Method:==== | ====Method:==== | ||
- | # | + | # Made up 25 Ampicillin plates using 400mL LB agar.(Using 100mg/ml Ampicillin stock from Gooley Lab). |
- | # | + | # Make up Kanamycin plates. |
+ | #[[Melbourne/IGEM 2007 kit|Resuspended the following from Registry plates]]. | ||
+ | #[[Melb:Transformation Protocol|Transformed]] into Joe's competent DH5alpha cells. | ||
+ | # Streak the cells onto plates. | ||
+ | #[[Melb:Growing up cells|Prepared]] in Amp LB (100ug/mL) or Kan LB (50mg/mL) as appropriate. | ||
+ | #[[Melb:Miniprep protocol|Minipreped]] the cultures grown overnight after putting aside 1mL(sterile) for glycerol stocks. | ||
+ | #[[Melbourne/Glycerol Stocks|made glycerol stocks]]. | ||
+ | #[[Melbourne/Preparing an agarose gel|Prepared an agarose gel]] with 1xTAE buffer. | ||
+ | # Digest | ||
+ | #[[Melbourne/Loading a DNA gel|Loaded]] 20uL of digest samples from 6/7 in the following lane order. | ||
====Results:==== | ====Results:==== | ||
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** | ** | ||
*Gells: | *Gells: | ||
- | + | <table> | |
- | + | <tr> | |
- | + | <td>[[Image:Melbourne_Experiment_2_Part_1.jpg|left|thumb|300px|Part 1]] | |
+ | </td> | ||
+ | <td>[[Image:Melbourne_Experiment_2_Part_2.jpg|none|thumb|300px|Part 2]] | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>[[Image:Melbourne_Experiment_2_Part_3.jpg|none|thumb|300px|Part 3]] | ||
+ | </td> | ||
+ | <td>[[Image:Melbourne_Experiment_2_part_4.jpg|none|thumb|300px|Part 4]] | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>[[Image:Melbourne_Experiment_2_part_5.jpg|none|thumb|300px|Part 5]] | ||
+ | </td> | ||
+ | </tr> | ||
+ | </table> | ||
+ | |||
+ | |||
====Conclusions:==== | ====Conclusions:==== |
Latest revision as of 03:44, 25 October 2007
<Return to lab notebook> <Back to team home page>
Contents |
Dates:
- Method Documentation Comenced:1 July 2007
- Method Documentation Completed:
- Author:
- Experiment Commenced:
- Experiment Completed:10 July 2007
- Write up Completed:
Aim:
- Prepare sufficient quantities of kit and other plasmids that are intended to be used in project.
- Confirm of contents of minipreped plasmids by digest.
Method:
- Made up 25 Ampicillin plates using 400mL LB agar.(Using 100mg/ml Ampicillin stock from Gooley Lab).
- Make up Kanamycin plates.
- Resuspended the following from Registry plates.
- Transformed into Joe's competent DH5alpha cells.
- Streak the cells onto plates.
- Prepared in Amp LB (100ug/mL) or Kan LB (50mg/mL) as appropriate.
- Minipreped the cultures grown overnight after putting aside 1mL(sterile) for glycerol stocks.
- made glycerol stocks.
- Prepared an agarose gel with 1xTAE buffer.
- Digest
- Loaded 20uL of digest samples from 6/7 in the following lane order.
Results:
- Jottings:
- Gells: