Melbourne/Blue Photosensor Background

From 2007.igem.org

(Difference between revisions)
Line 42: Line 42:
[[Melbourne/Blue Photosensor Background/ComP/AA|AA]],
[[Melbourne/Blue Photosensor Background/ComP/AA|AA]],
[http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=search&term=sopII genbank]
[http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=search&term=sopII genbank]
 +
[[Melbourne/primary_Restriction_enzymes|SpeI]] (@927) deleted
[[Melbourne/primary_Restriction_enzymes|SpeI]] (@927) deleted
[[Melbourne/primary_Restriction_enzymes|HaeII]] (@1902) inserted
[[Melbourne/primary_Restriction_enzymes|HaeII]] (@1902) inserted
Line 53: Line 54:
[[Melbourne/Blue Photosensor Background/ComA/AA|AA]],
[[Melbourne/Blue Photosensor Background/ComA/AA|AA]],
[http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=search&term=sopII genbank]
[http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=search&term=sopII genbank]
 +
[[Melbourne/primary_Restriction_enzymes|SpeI]] (@12) deleted
[[Melbourne/primary_Restriction_enzymes|SpeI]] (@12) deleted
[[Melbourne/primary_Restriction_enzymes|HaeII]] (@433) inserted
[[Melbourne/primary_Restriction_enzymes|HaeII]] (@433) inserted

Revision as of 11:50, 6 August 2007

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Contents

Preliminaries

SopII

sensory rhodopsin II

DNA, AA, [http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=search&term=sopII genbank]

  • No IGEM restriction sites
  • HaeII (@168) present


HtrII

sensory rhodopsin II transducer

DNA, AA, [http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=3702851&ordinalpos=1&itool=EntrezSystem2.PEntrez.Gene.Gene_ResultsPanel.Gene_RVDocSum genbank]

  • No IGEM restriction sites
  • No useful restriction site


SopII/HtrII fusion

DNA, AA (frame 2 in Translate - only SopII + HtrII + Linker included in link), linker = TSASA SNGASA,

[http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=search&term=sopII genbank]

  • No IGEM restriction sites
  • Use site from SopII (this is the one that will be used)


ComP

two-component sensor histidine kinase

DNA (includes IGEM forward and reverse primers (VF2 + VR) and IGEM prefix and suffix, AA, [http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=search&term=sopII genbank]

SpeI (@927) deleted HaeII (@1902) inserted


ComA

sensory rhodopsin II transducer

DNA, AA, [http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=search&term=sopII genbank]

SpeI (@12) deleted HaeII (@433) inserted

File:Seq align.jpg Media:Example.ogg