Melbourne/Blue Photosensor Background
From 2007.igem.org
(Difference between revisions)
| Line 3: | Line 3: | ||
====Preliminaries==== | ====Preliminaries==== | ||
SopII - sensory rhodopsin II | SopII - sensory rhodopsin II | ||
| - | [[Melbourne/Blue Photosensor Background/SopII/DNA|DNA | + | [[Melbourne/Blue Photosensor Background/SopII/DNA|DNA]], |
| - | [[Melbourne/Blue Photosensor Background/SopII/AA|AA | + | [[Melbourne/Blue Photosensor Background/SopII/AA|AA]] |
[http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=search&term=sopII genbank] | [http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=search&term=sopII genbank] | ||
| + | * No IGEM restriction sites | ||
| + | * HaeII site at bp - 168 | ||
HtrII - sensory rhodopsin II transducer | HtrII - sensory rhodopsin II transducer | ||
| - | [[Melbourne/Blue Photosensor Background/ | + | [[Melbourne/Blue Photosensor Background/HtrIIA|DNA]], |
| - | [[Melbourne/Blue Photosensor Background/ | + | [[Melbourne/Blue Photosensor Background/HtrII/AA|AA]] |
| - | [http://www.ncbi.nlm.nih.gov/sites/entrez? | + | [http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=3702851&ordinalpos=1&itool=EntrezSystem2.PEntrez.Gene.Gene_ResultsPanel.Gene_RVDocSum genbank] |
| - | + | * No IGEM restriction sites | |
| + | * No useful restriction site | ||
SopII/HtrII fusion | SopII/HtrII fusion | ||
| - | [[Melbourne/Blue Photosensor Background/Fusion/DNA|DNA | + | [[Melbourne/Blue Photosensor Background/Fusion/DNA|DNA]], |
| - | [[Melbourne/Blue Photosensor Background/Fusion/AA|AA | + | [[Melbourne/Blue Photosensor Background/Fusion/AA|AA (frame 2 in Translate)]] |
[http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=search&term=sopII genbank] | [http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=search&term=sopII genbank] | ||
| + | * No IGEM restriction sites | ||
| + | * Use site from SopII (this is the one that will be used) | ||
| + | |||
ComP - two-component sensor histidine kinase | ComP - two-component sensor histidine kinase | ||
| - | [[Melbourne/Blue Photosensor Background/ | + | [[Melbourne/Blue Photosensor Background/ComP/DNA|DNA]], |
| - | [[Melbourne/Blue Photosensor Background/ | + | [[Melbourne/Blue Photosensor Background/ComP/AA|AA]] |
[http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=search&term=sopII genbank] | [http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=search&term=sopII genbank] | ||
| + | [[Melbourne/primary_Restriction_enzymes|SpeI]] (@927) deleted | ||
| + | [[Melbourne/primary_Restriction_enzymes|HaeII]] (@1902) inserted | ||
| + | |||
ComA - sensory rhodopsin II transducer | ComA - sensory rhodopsin II transducer | ||
| - | [[Melbourne/Blue Photosensor Background/ | + | [[Melbourne/Blue Photosensor Background/ComA/DNA|DNA]], |
| - | [[Melbourne/Blue Photosensor Background/ | + | [[Melbourne/Blue Photosensor Background/ComA/AA|AA]] |
[http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=search&term=sopII genbank] | [http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=search&term=sopII genbank] | ||
| + | [[Melbourne/primary_Restriction_enzymes|SpeI]] (@12) deleted | ||
| + | [[Melbourne/primary_Restriction_enzymes|HaeII]] (@433) inserted | ||
[[Image:seq_align.jpg]] | [[Image:seq_align.jpg]] | ||
[[Media:Example.ogg]] | [[Media:Example.ogg]] | ||
Revision as of 11:19, 6 August 2007
<return to top of background> <return to home page>
Preliminaries
SopII - sensory rhodopsin II DNA, AA genbank
- No IGEM restriction sites
- HaeII site at bp - 168
HtrII - sensory rhodopsin II transducer DNA, AA genbank
- No IGEM restriction sites
- No useful restriction site
SopII/HtrII fusion DNA, AA (frame 2 in Translate) genbank
- No IGEM restriction sites
- Use site from SopII (this is the one that will be used)
ComP - two-component sensor histidine kinase
DNA,
AA
genbank
SpeI (@927) deleted
HaeII (@1902) inserted
ComA - sensory rhodopsin II transducer
DNA,
AA
genbank
SpeI (@12) deleted
HaeII (@433) inserted
File:Seq align.jpg Media:Example.ogg
