Melbourne/Blue Photosensor Background
From 2007.igem.org
(Difference between revisions)
| Line 30: | Line 30: | ||
ComP - two-component sensor histidine kinase | ComP - two-component sensor histidine kinase | ||
| - | [[Melbourne/Blue Photosensor Background/ComP/DNA|DNA]], | + | [[Melbourne/Blue Photosensor Background/ComP/DNA|DNA(includes IGEM forward and reverse primers (VF2 + VR) and IGEM prefix and suffix]], |
[[Melbourne/Blue Photosensor Background/ComP/AA|AA]] | [[Melbourne/Blue Photosensor Background/ComP/AA|AA]] | ||
[http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=search&term=sopII genbank] | [http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=search&term=sopII genbank] | ||
Revision as of 11:36, 6 August 2007
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Preliminaries
SopII - sensory rhodopsin II DNA, AA genbank
- No IGEM restriction sites
- HaeII (@168) present
HtrII - sensory rhodopsin II transducer
DNA,
AA
genbank
- No IGEM restriction sites
- No useful restriction site
SopII/HtrII fusion
DNA,
AA (frame 2 in Translate - only SopII + HtrII + Linker included in link), linker = TSASA
SNGASA
- No IGEM restriction sites
- Use site from SopII (this is the one that will be used)
ComP - two-component sensor histidine kinase
DNA(includes IGEM forward and reverse primers (VF2 + VR) and IGEM prefix and suffix,
AA
genbank
SpeI (@927) deleted
HaeII (@1902) inserted
ComA - sensory rhodopsin II transducer
DNA,
AA
genbank
SpeI (@12) deleted
HaeII (@433) inserted
File:Seq align.jpg Media:Example.ogg
