Melbourne/Blue Photosensor Background
From 2007.igem.org
(Difference between revisions)
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[[Melbourne/Background|<return to top of background>]] [[melbourne|<return to home page>]] | [[Melbourne/Background|<return to top of background>]] [[melbourne|<return to home page>]] | ||
| - | + | ==Preliminaries== | |
| - | == SopII == | + | ==== SopII ==== |
sensory rhodopsin II | sensory rhodopsin II | ||
[[Melbourne/Blue Photosensor Background/SopII/DNA|DNA]], | [[Melbourne/Blue Photosensor Background/SopII/DNA|DNA]], | ||
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| - | == HtrII == | + | ==== HtrII ==== |
sensory rhodopsin II transducer | sensory rhodopsin II transducer | ||
[[Melbourne/Blue Photosensor Background/HtrIIA|DNA]], | [[Melbourne/Blue Photosensor Background/HtrIIA|DNA]], | ||
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| - | == | + | ==== SopII/HtrII fusion ==== |
| - | SopII/HtrII fusion == | + | |
[[Melbourne/Blue Photosensor Background/Fusion/DNA|DNA]], | [[Melbourne/Blue Photosensor Background/Fusion/DNA|DNA]], | ||
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| - | == ComP == | + | ==== ComP ==== |
two-component sensor histidine kinase | two-component sensor histidine kinase | ||
[[Melbourne/Blue Photosensor Background/ComP/DNA|DNA]] (includes IGEM forward and reverse primers (VF2 + VR) and IGEM prefix and suffix, | [[Melbourne/Blue Photosensor Background/ComP/DNA|DNA]] (includes IGEM forward and reverse primers (VF2 + VR) and IGEM prefix and suffix, | ||
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| - | == ComA == | + | ==== ComA ==== |
sensory rhodopsin II transducer | sensory rhodopsin II transducer | ||
[[Melbourne/Blue Photosensor Background/ComA/DNA|DNA]], | [[Melbourne/Blue Photosensor Background/ComA/DNA|DNA]], | ||
Revision as of 11:38, 6 August 2007
<return to top of background> <return to home page>
Contents |
Preliminaries
SopII
sensory rhodopsin II DNA, AA genbank
- No IGEM restriction sites
- HaeII (@168) present
HtrII
sensory rhodopsin II transducer DNA, AA genbank
- No IGEM restriction sites
- No useful restriction site
SopII/HtrII fusion
DNA, AA (frame 2 in Translate - only SopII + HtrII + Linker included in link), linker = TSASA SNGASA
- No IGEM restriction sites
- Use site from SopII (this is the one that will be used)
ComP
two-component sensor histidine kinase DNA (includes IGEM forward and reverse primers (VF2 + VR) and IGEM prefix and suffix, AA genbank SpeI (@927) deleted HaeII (@1902) inserted
ComA
sensory rhodopsin II transducer DNA, AA genbank SpeI (@12) deleted HaeII (@433) inserted
File:Seq align.jpg Media:Example.ogg
