Melbourne/Blue Photosensor Background

From 2007.igem.org

(Difference between revisions)
Line 5: Line 5:
==== SopII ====
==== SopII ====
sensory rhodopsin II  
sensory rhodopsin II  
 +
[[Melbourne/Blue Photosensor Background/SopII/DNA|DNA]],
[[Melbourne/Blue Photosensor Background/SopII/DNA|DNA]],
[[Melbourne/Blue Photosensor Background/SopII/AA|AA]]
[[Melbourne/Blue Photosensor Background/SopII/AA|AA]]
Line 15: Line 16:
==== HtrII ====
==== HtrII ====
sensory rhodopsin II transducer
sensory rhodopsin II transducer
 +
[[Melbourne/Blue Photosensor Background/HtrIIA|DNA]],
[[Melbourne/Blue Photosensor Background/HtrIIA|DNA]],
[[Melbourne/Blue Photosensor Background/HtrII/AA|AA]]
[[Melbourne/Blue Photosensor Background/HtrII/AA|AA]]
Line 36: Line 38:
==== ComP ====
==== ComP ====
two-component sensor histidine kinase
two-component sensor histidine kinase
 +
[[Melbourne/Blue Photosensor Background/ComP/DNA|DNA]] (includes IGEM forward and reverse primers (VF2 + VR) and IGEM prefix and suffix,
[[Melbourne/Blue Photosensor Background/ComP/DNA|DNA]] (includes IGEM forward and reverse primers (VF2 + VR) and IGEM prefix and suffix,
[[Melbourne/Blue Photosensor Background/ComP/AA|AA]]
[[Melbourne/Blue Photosensor Background/ComP/AA|AA]]
Line 46: Line 49:
==== ComA ====
==== ComA ====
sensory rhodopsin II transducer
sensory rhodopsin II transducer
 +
[[Melbourne/Blue Photosensor Background/ComA/DNA|DNA]],
[[Melbourne/Blue Photosensor Background/ComA/DNA|DNA]],
[[Melbourne/Blue Photosensor Background/ComA/AA|AA]]
[[Melbourne/Blue Photosensor Background/ComA/AA|AA]]

Revision as of 11:39, 6 August 2007

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Contents

Preliminaries

SopII

sensory rhodopsin II

DNA, AA genbank

  • No IGEM restriction sites
  • HaeII (@168) present


HtrII

sensory rhodopsin II transducer

DNA, AA genbank

  • No IGEM restriction sites
  • No useful restriction site


SopII/HtrII fusion

DNA, AA (frame 2 in Translate - only SopII + HtrII + Linker included in link), linker = TSASA SNGASA

genbank

  • No IGEM restriction sites
  • Use site from SopII (this is the one that will be used)


ComP

two-component sensor histidine kinase

DNA (includes IGEM forward and reverse primers (VF2 + VR) and IGEM prefix and suffix, AA genbank SpeI (@927) deleted HaeII (@1902) inserted


ComA

sensory rhodopsin II transducer

DNA, AA genbank SpeI (@12) deleted HaeII (@433) inserted

File:Seq align.jpg Media:Example.ogg