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Revision as of 11:50, 6 August 2007 (view source) Honza83 (Talk | contribs) ← Older edit		Revision as of 11:52, 6 August 2007 (view source) Honza83 (Talk | contribs) Newer edit →
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	[[Melbourne/primary_Restriction_enzymes|SpeI]] (@12) deleted		[[Melbourne/primary_Restriction_enzymes|SpeI]] (@12) deleted
	[[Melbourne/primary_Restriction_enzymes|HaeII]] (@433) inserted		[[Melbourne/primary_Restriction_enzymes|HaeII]] (@433) inserted
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	[[Image:seq_align.jpg]]		[[Image:seq_align.jpg]]
	[[Media:Example.ogg]]		[[Media:Example.ogg]]

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Revision as of 11:52, 6 August 2007

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Contents 1 Preliminaries 1.1 SopII 1.2 HtrII 1.3 SopII/HtrII fusion 1.4 ComP 1.5 ComA

Preliminaries

SopII

sensory rhodopsin II

DNA, AA, genbank

• No IGEM restriction sites
• HaeII (@168) present

HtrII

sensory rhodopsin II transducer

DNA, AA, genbank

• No IGEM restriction sites
• No useful restriction site

SopII/HtrII fusion

DNA, AA (frame 2 in Translate - only SopII + HtrII + Linker included in link), linker = TSASA SNGASA,

genbank

• No IGEM restriction sites
• Use site from SopII (this is the one that will be used)

ComP

two-component sensor histidine kinase

DNA (includes IGEM forward and reverse primers (VF2 + VR) and IGEM prefix and suffix, AA, genbank

SpeI (@927) deleted HaeII (@1902) inserted

ComA

sensory rhodopsin II transducer

DNA, AA, genbank

SpeI (@12) deleted HaeII (@433) inserted

File:Seq align.jpg Media:Example.ogg