Melbourne/Blue Photosensor Background
From 2007.igem.org
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Preliminaries
SopII - sensory rhodopsin II DNA, AA genbank
- No IGEM restriction sites
- HaeII (@168) present
HtrII - sensory rhodopsin II transducer
DNA,
AA
genbank
- No IGEM restriction sites
- No useful restriction site
SopII/HtrII fusion
DNA,
AA (frame 2 in Translate - only SopII + HtrII + Linker included in link), linker = TSASA
SNGASA
- No IGEM restriction sites
- Use site from SopII (this is the one that will be used)
ComP - two-component sensor histidine kinase
DNA(includes IGEM forward and reverse primers (VF2 + VR) and IGEM prefix and suffix,
AA
genbank
SpeI (@927) deleted
HaeII (@1902) inserted
ComA - sensory rhodopsin II transducer
DNA,
AA
genbank
SpeI (@12) deleted
HaeII (@433) inserted
File:Seq align.jpg Media:Example.ogg