Melbourne/Blue Photosensor Background
From 2007.igem.org
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Contents |
Preliminaries
== SopII ==
- sensory rhodopsin II
- No IGEM restriction sites
- HaeII (@168) present
HtrII
- sensory rhodopsin II transducer
- No IGEM restriction sites
- No useful restriction site
==
SopII/HtrII fusion ==
DNA, AA (frame 2 in Translate - only SopII + HtrII + Linker included in link), linker = TSASA SNGASA
- No IGEM restriction sites
- Use site from SopII (this is the one that will be used)
ComP
- two-component sensor histidine kinase
DNA (includes IGEM forward and reverse primers (VF2 + VR) and IGEM prefix and suffix, AA genbank SpeI (@927) deleted HaeII (@1902) inserted
ComA
- sensory rhodopsin II transducer
DNA, AA genbank SpeI (@12) deleted HaeII (@433) inserted
File:Seq align.jpg Media:Example.ogg