From 2007.igem.org

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Contents 1 Preliminaries 2 HtrII 3 ComP 4 ComA

Preliminaries

== SopII == sensory rhodopsin II DNA, AA genbank

• No IGEM restriction sites
• HaeII (@168) present

HtrII

sensory rhodopsin II transducer DNA, AA genbank

• No IGEM restriction sites
• No useful restriction site

== SopII/HtrII fusion ==

DNA, AA (frame 2 in Translate - only SopII + HtrII + Linker included in link), linker = TSASA SNGASA

genbank

• No IGEM restriction sites
• Use site from SopII (this is the one that will be used)

ComP

two-component sensor histidine kinase DNA (includes IGEM forward and reverse primers (VF2 + VR) and IGEM prefix and suffix, AA genbank SpeI (@927) deleted HaeII (@1902) inserted

ComA

sensory rhodopsin II transducer DNA, AA genbank SpeI (@12) deleted HaeII (@433) inserted

File:Seq align.jpg Media:Example.ogg