Melbourne/DNA concentration measurement
From 2007.igem.org
< Melbourne(Difference between revisions)
| Line 22: | Line 22: | ||
#Pipette into an acrylamide cuvette the contents of the eppendorf. | #Pipette into an acrylamide cuvette the contents of the eppendorf. | ||
#Insert the cuvette into the spectrometer. | #Insert the cuvette into the spectrometer. | ||
| - | #Push "sample" to measure the | + | #Push "sample" to measure the interference. |
#Record the optical density (OD). | #Record the optical density (OD). | ||
#Calculate the concentration of DNA in the original form. | #Calculate the concentration of DNA in the original form. | ||
| - | + | <i> 1OD=50ng/ul. Thereofe, for example, if the OD is 0.005, the concentration in the original form is 0.005 X 200 X 50 = 50ng/ul.</i> | |
=====Equipement Required===== | =====Equipement Required===== | ||
*[[Melbourne/1.7ml microcentrifuge|microcentrifuge: 1.7ml]] | *[[Melbourne/1.7ml microcentrifuge|microcentrifuge: 1.7ml]] | ||
*Pipettes | *Pipettes | ||
| - | * | + | *Acrylamide cuvette |
| - | + | *Vortex | |
| + | *Spectrophotometer | ||
=====References===== | =====References===== | ||
* | * | ||
Latest revision as of 15:54, 28 September 2007
<Return to list of protocols> <Team home page>
- Applications: Measure the concentration of DNA in solution
- Time to complete protocol: 5 min
- Lab time: 5 minutes
- Waiting time: 0
- Approximate cost of materials: $0
Contents |
Method from primary and secondary reagents
Primary & secondary Reagents Required including controls
- DNA to be measured
- milliQ water
Method including controls
- Set the spectrometer to measure dsDNA (260nm)
- Insert into an acrylamide cuvette 1ml of milliQ water
- Insert the cuvette into the spectrometer and push "set reference"
- Take the cuvette out of the spectrometer and empty the contents.
- To an eppendorf tube, add 5ul of DNA.
- Add 1ml of milliQ water to the eppendorf tube.
- Vortex
- Pipette into an acrylamide cuvette the contents of the eppendorf.
- Insert the cuvette into the spectrometer.
- Push "sample" to measure the interference.
- Record the optical density (OD).
- Calculate the concentration of DNA in the original form.
1OD=50ng/ul. Thereofe, for example, if the OD is 0.005, the concentration in the original form is 0.005 X 200 X 50 = 50ng/ul.
Equipement Required
- microcentrifuge: 1.7ml
- Pipettes
- Acrylamide cuvette
- Vortex
- Spectrophotometer
