Paris/July 16
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* Either the fluorescence without IPTG is due to a leak of the promoter | * Either the fluorescence without IPTG is due to a leak of the promoter | ||
* Either DGAT is not induce in presence of IPTG, and the fluorescence we see is only a background. | * Either DGAT is not induce in presence of IPTG, and the fluorescence we see is only a background. | ||
| + | == Growth kinetics of w121 strain == | ||
| + | Results of the previous day : we lost everything because of a crash of the computer :(. | ||
Revision as of 14:38, 16 July 2007
Plasmid pKs::DGAT expression in E. Coli
We tried to see TG with NR died on E. Coli cells transfected with pKs::DGAT, an IPTG-inducible promoter.
We tried different growth media containing more or less oleate, that should in theory increase TG synthesis.
Results : we don't see the inductible effect of IPTG. We can think that :
- Either the fluorescence without IPTG is due to a leak of the promoter
- Either DGAT is not induce in presence of IPTG, and the fluorescence we see is only a background.
Growth kinetics of w121 strain
Results of the previous day : we lost everything because of a crash of the computer :(.
