Paris/July 4
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== Low Nitrogen Minimum Medium with Nile Red == | == Low Nitrogen Minimum Medium with Nile Red == | ||
| - | + | See [[Paris/PROTOCOLS|protocols]]. | |
[[Paris|<<home]] | [[Paris|<<home]] | ||
Revision as of 12:09, 20 July 2007
Contents |
Transduction to MG1655 using the P1 stock made on w121.
See protocols.
Growth kinetics : Results of July, 3
Growth of DapA- strain (w121) relative to the concentration of DAP in the medium
Measuring excretion of Dap by MG1655 DapA+ strain in an indirect manner
The w121 did not grow at all in the S0 medium... Either we did something wrong, or their is a logical explanation ! There might be growth inhibitors in the ON medium, their might be no nutriments left in the medium...
We will redo the experiment but this time, the medium will be recovered from exponential phase culture of MG1655
Acinetobacter liquid culture
We picked up a colony from LB solid culture from 07/03/2007 and put it into 5mL LB medium (30°C aerobic).
Low Nitrogen Minimum Medium with Nile Red
See protocols.
