Paris/October 19

From 2007.igem.org

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(Digestion reactions)
(Digestion reactions)
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|rbsdapAcoli BI
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|rbsdapAsubtilis BI
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|L64
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Revision as of 14:07, 19 October 2007

Digestion reactions

Digestion Products
Number Product Name Matrix Name Enzyme 1 Enzyme 2 Size Description
D22bis pSB1A2 BBv MP6 EcoRI PstI pSB1A2 vector digested with EcoRI and PstI enzymes
D76 DGAT BB P14(19/10/07) EcoRI PstI DGAT (delta PstI)
D79 DGAT BI P14(19/10/07) XbaI PstI DGAT(delta PstI)PCR product for cloming as a BB in D47
D80 PacI[ptet>>lox71-gfpT-lox66-mrfp]SmaI P15 PacI SmaI ptet>>lox71-gfpT-lox66-mrfp P15 PCR product digested by PacI-SmaI for cloning into pY-2C-IntC
D81 PacI[pY-2C-IntC]SmaI pY-2C-IntC plasmid PacI SmaI pY-2C-IntC digested for ptet>>lox71-gfpT-lox66-mrfp cloning
D82 frt1-CmR-frt2 FI P16 EcoRI SpeI frt1-CmR-frt2 PCR product digested for use as a FI
D83 frt1-CmR-frt2 BI P16 XbaI PstI frt1-CmR-frt2 PCR product digested for use as a BI
D84 frt1-CmR-frt2 BBi P16 EcoRI PstI frt1-CmR-frt2 PCR product digested for cloning as a BB
D85 frt1-CmR-frt2-T1T2 FI P17 EcoRI SpeI frt1-CmR-frt2-T1T2 PCR product digested for use as a FI
D86 frt1-CmR-frt2-T1T2 BI P17 XbaI- PstI frt1-CmR-frt2-T1T2 PCR product digested for use as a BI
D87 frt1-CmR-frt2-T1T2 BBi P17 EcoRI PstI frt1-CmR-frt2-T1T2 PCR product digested for cloning as a BB
D88 pTet>>lox71-gfptripqrt FV L34 EcoRI XbaI pTet>>lox71-gfptripqrt pSB1A2 dig. for use as a FV
D89 T-lox66-rbsdapAcoli BV L53 SpeI PstI T-lox66-rbsdapAcoli pSB1A2 dig. for use as a BV
D90 T-lox66-rbsdapAsubtilis BV L56 SpeI PstI T-lox66-rbsdapAsubtilis pSB1A2 dig. for use as a BV
D91 ptet>>lox71-gfpT-lox66-mrfp pSB1A2 BV L59 SpeI PstI ptet>>lox71-gfpT-lox66-mrfp pSB1A2 dig. for use as a BV
D92 rbsdapAcoli BI L39 XbaI PstI rbsdapAcoli extracted for use as a BI
D93 rbsdapAsubtilis BI L64 XbaI PstI rbsdapAsubtilis extracted for use as a BI