Table of Oligos
|
Number
| Name
| Sequence
| Length
| Comments
|
1
| Lox71-FtsZ-F
| TTTGAATTCGCGGCCGCTTCTAGAGTACCGTTCGTATACGATACATT ATACGAAGTTATTAATAAACAGGCACAAATCGGAGAGAAACT
| 89
|
|
2
| FtsZ-R
| TTTCTGCAGCGGCCGCTACTAGTATTTTTAATCAGCTTGCTTACGCAGGAAT
| 49
|
|
3
| Lox71-FtsA-F
| TTTGAATTCGCGGCCGCTTCTAGAGTACCGTTCGTATACGATACATTATACG AAGTTATTAATAATCAGGCACAGGCAGAACAACAATG
| 89
|
|
4
| DEcoR1-FtsZ-R
| CGGTATTTACCGCGAAGAACTCAACACCTTCAATGCGCTC
| 40
|
|
5
| DEcoR1-FtsZ-F
| TGCGCGAGCGCATTGAAGGTGTTGAGTTCTTCGCGGTAAA
| 40
|
|
6
| Lox66-DapAColi-F
| TTTGAATTCGCGGCCGCTTCTAGAGATAACTTGGTATAGCATACATTATACG AACGGTAGTTTGCACAGAGGATGGCCCATGTT
| 84
|
|
7
| DapAColi-R
| TTTCTGCAGCGGCCGCTACTAGTATTATTACAGCAAACCGGCATGCTTAAGC
| 52
|
|
8
| Lox66-DapASubtilis-F
| TTTGAATTCGCGGCCGCTTCTAGAGATAACTTGGTATAGCATACATTATACG AACGGTAATCATGATGAGGTGAATAAAATGAA
| 84
|
|
9
| DapASubtilis-R
| TTTCTGCAGCGGCCGCTACTAGTATTATTACAGTTCGCTGATCGTGCTGCTT
| 52
|
|
10
| Lox71-KmR-F
| TTTCTCTAGAAGCGGCCGCGAATTCTACCGTTCGTATACGATACATTATACG AAGTTATCTGATCCTTCAACTCAGCAAAAGTTC
| 85
|
|
11
| Lox66-KmR-R
| TTTTACTAGTAGCGGCCGCTGCAGTACCGTTCGTATAATGTATGCTATACCA AGTTATTTATTAGAAAAACTCATCGAGCATCAAA
| 86
|
|
12
| DPst1-DGAT-F
| AGTGCTGTCGTATATGGCCCAGCAGGACTCAACATAATTT
| 40
|
|
13
| DPst1-DGAT-R
| AAATTATGTTGAGTCCTGCTGGGCCATATACGACAGCACT
| 40
|
|
14
| Lox66-F
| AATTCGCGGCCGCTTCTAGAGATAACTTGGTATAGCATACATTATACGAACGGTATAC TAGTAGCGGCCGCTGCA
|
|
|
15
| Lox66-R
| GCGGCCGCTACTAGTATACCGTTCGTATAATGTATGCTATACCAAGTTATCTCTAG AAGCGGCCGCG
|
|
|
16
| Lox71-F
| AATTCGCGGCCGCTTCTAGAGTACCGTTCGTATACGATACATTATACGAAGTTATTACT AGTAGCGGCCGCTGCA
|
|
|
17
| Lox71-R
| GCGGCCGCTACTAGTAATAACTTCGTATAATGTATCGTATACGAACGGTACTCTAG AAGCGGCCGCG
|
|
|
18
| VF2
| tgccacctgacgtctaagaa
| 20
| Forward Primer to Biobrick vectors (VF2) BBa_G00100
|
19
| VR
| attaccgcctttgagtgagc
| 20
| Reverse Primer to Biobrick Vectors (VR) BBa_G00101
|
20
| RBS-DapAColi-F
| TTTGAATTCGCGGCCGCTTCTAGAGATTAAAGAGGAGAAATACTAGATGTT CACGGGAAGTATTGTCGCGA
| 71
| Biobrick prefix + Strong RBS BBa_B0030 + 25 bp homology to DapA (Coli) from ATG
|
21
| RBS-DapASubtilis-F
| TTTGAATTCGCGGCCGCTTCTAGAGATTAAAGAGGAGAAATACTAGATGAA TTTCGGAAATGTGTCTACAG
| 71
| Biobrick prefix + Strong RBS BBa_B0030 + 25 bp homology to DapA (Subtilis) from ATG
|
22
| SEQFtsA-1F
| GTCGTCCATACCGCGAAATC
| 20
|
|
23
| SEQFtsA-2F
| CGTGGAAGTGCCGAGCGTAG
| 20
|
|
24
| SEQFtsZ-1F
| GGTATCGCTGAACTGATTAC
| 20
|
|
25
| SEQDapASub-1F
| TTAAAGCAATTGCGGCAGAG
| 20
|
|
26
| SEQDapAColi-1F
| CAATCGTCCGTCGCAAGAAG
| 20
|
|
27
| ForDGAT1
| ATCCACGCTATGCGCCCATTAC
| 22
| Tm = 60°C
|
28
| RevDGAT1
| GGGCTAATTTAGCCCTTTAGTT
| 22
| Tm = 50°C
|
29
| ForDGAT_BB1
| AAAGAATTCGCGGCCGCTTCTAGAGATTAAAGAGGAGAAAATCCACGCTATGCGCCCATTAC
| 62
| Tm = 60/83°C
|
30
| RevDGAT_BB1
| CTGCAGCGGCCGCTACTAGTAGGGCTAATTTAGCCCTTTAGTT
| 43
| Tm = 50/74°C
|
31
| DapAp-F
| aattcgcggccgcttctagagAACAATCAGAACGGTTCTGTCTGCTTGCTTTT AATGCCATACCAAACGTACCATTGAGACACTTGTTTGCACAGAGGATGGta
| 104
|
|
32
| DapAp-R
| ctagtaCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCA TTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTctctagaagcggccgcg
| 104
|
|
33
| dDapA-F
| CATACCAAACGTACCATTGAGACACTTGTTTGCACAGAGGAT GGCCCATGATTCCGGGGATCCGTCGACC
| 70
| more here
|
34
| dDapA-R
| AAGCCATCAAATCTCCCTAAACTTTACAGCAAACCGGCATGC TTAAGCGCTGTAGGCTGGAGCTGCTTCG
| 70
| more here
|
35
| dFtsZ-F
| GACGATGATTACGGCCTCAGGCGACAGGCACAAATCGGAGAGA AACTATGATTCCGGGGATCCGTCGACC
| 70
| more here
|
36
| dFtsZ-R
| GAAACCCAAATTCCAGTCAATTCTTAATCAGCTTGCTTACGCA GGAATGCTGTAGGCTGGAGCTGCTTCG
| 70
| more here
|
37
| dDapAverif-F
| ACAATCAGAACGGTTCTGTCTG
| 22
|
|
38
| dDapAverif-R
| ATAGCGTGAGTCAGAACTACAG
| 22
|
|
39
| dFtsZverif-R
| TCGCCAAATTACCTATCCAACC
| 22
|
|