PennState/Parts

From 2007.igem.org

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<a href="PART LINK HERE"><img border="0" src="http://openwetware.org/images/3/3e/Psuigem07picGene.jpg" width="121" height="121"></a></td>
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<td class="tddesc"><b><a href="http://partsregistry.org/Part:BBa_I741023">CRP*</a></b><p><i>CRP* is a modified CRP which is cAMP independent. CRP is a dual transcriptional regulator that induces a severe bend in the DNA and is capable of regulating the expression of more than 200 genes. It is the major global regulator of catabolite-sensitive operons and it controls its own synthesis.<a href="http://biocyc.org/ECOLI/NEW-IMAGE?type=ENZYME&object=CPLX0-226">[1]</a></i></td>
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<td class="tddesc"><b><a href="http://partsregistry.org/Part:BBa_I741023">CRP*</a></b><p><i>CRP* is a modified CRP which is cAMP independent. CRP is a dual transcriptional regulator that induces a severe bend in the DNA and is capable of regulating the expression of more than 200 genes. It is the major global regulator of catabolite-sensitive operons and it controls its own synthesis.</i><a href="http://biocyc.org/ECOLI/NEW-IMAGE?type=ENZYME&object=CPLX0-226">[1]</a></td>
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Revision as of 20:32, 26 October 2007

Home Diauxie Project Dosimeter Project People Lab Parts Acknowledgements Links


New Parts (submitted)

xylR

XylR is a protein that belongs to the AraC/XylS family. This regulator participates in controlling several genes involved in xylose utilization. XylR activates the xylAB and xylFGHR operons.

Promoter xylF

The xylose F promoter is activated by xylR-xylose, and may be negatively regulated by xylR. Two xylR halfsites are included as well as a CRP activator binding site.



New Parts (underconstructed/unverified)

natural RBS xylR TT 'xylR total'

XylR is a protein that belongs to the AraC/XylS family. This regulator participates in controlling several genes involved in xylose utilization. XylR activates the xylAB and xylFGHR operons.

CRP*

CRP* is a modified CRP which is cAMP independent. CRP is a dual transcriptional regulator that induces a severe bend in the DNA and is capable of regulating the expression of more than 200 genes. It is the major global regulator of catabolite-sensitive operons and it controls its own synthesis.[1]

Promoter xylA

The xylose A promoter is activated by xylR-xylose, and may be negatively regulated by xylR. Two xylR halfsites are included as well as a CRP activator binding site.

Promoter xylA without CRP site

The xylose A promoter is activated by xylR-xylose, and may be negatively regulated by xylR. Two xylR halfsites are included, but the promoter region was cloned without the CRP binding site. Note: the removal of CRP may keep the promoter from working.

Promoter xylF without CRP site

The xylose A promoter is activated by xylR-xylose, and may be negatively regulated by xylR. Two xylR halfsites are included, but the promoter region was cloned without the CRP binding site. Note: the removal of CRP may keep the promoter from working.

Two way promoter xylA-F with CRP site

The xylose A promoter is activated by xylR-xylose, and may be negatively regulated by xylR. Two xylR halfsites are included and a CRP binding site.