PennState/Parts

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New Parts (submitted)

xylR

XylR is a protein that belongs to the AraC/XylS family. This regulator participates in controlling several genes involved in xylose utilization. XylR activates the xylAB and xylFGHR operons.[BioCyc.org]

Promoter xylF

The xylose F promoter is activated by xylR-xylose, and may be negatively regulated by xylR. Two xylR halfsites are included as well as a CRP activator binding site.[BioCyc.org]



New Parts (underconstructed/unverified)

natural RBS xylR TT 'xylR total'

XylR is a protein that belongs to the AraC/XylS family. This regulator participates in controlling several genes involved in xylose utilization. XylR activates the xylAB and xylFGHR operons.

CRP*

CRP* is a modified CRP which is cAMP independent. CRP is a dual transcriptional regulator that induces a severe bend in the DNA and is capable of regulating the expression of more than 200 genes. It is the major global regulator of catabolite-sensitive operons and it controls its own synthesis.[Hanamura92]

Promoter xylA

The xylose A promoter is activated by xylR-xylose, and may be negatively regulated by xylR. Two xylR halfsites are included as well as a CRP activator binding site.

Promoter xylA without CRP site

The xylose A promoter is activated by xylR-xylose, and may be negatively regulated by xylR. Two xylR halfsites are included, but the promoter region was cloned without the CRP binding site. Note: the removal of CRP may keep the promoter from working.

Promoter xylF without CRP site

The xylose A promoter is activated by xylR-xylose, and may be negatively regulated by xylR. Two xylR halfsites are included, but the promoter region was cloned without the CRP binding site. Note: the removal of CRP may keep the promoter from working.

Two way promoter xylA-F with CRP site

The xylose A promoter is activated by xylR-xylose, and may be negatively regulated by xylR. Two xylR halfsites are included and a CRP binding site.



References

Hanamura92: Hanamura A, Aiba H (1992). "A new aspect of transcriptional control of the Escherichia coli crp gene: positive autoregulation." Mol Microbiol 1992;6(17);2489-97. PMID: 1328816