Purdue

From 2007.igem.org

(Difference between revisions)
(Undergraduate Students)
(Abstract)
Line 1: Line 1:
== Abstract ==
== Abstract ==
-
This year's Purdue 2007 iGEM team will be preparing a project called "Bacteria
+
This year's Purdue 2007 iGEM team will be presenting a project called "Bacteria
Warfare" for this year's competition hosted by MIT in November. The team is
Warfare" for this year's competition hosted by MIT in November. The team is
composed of a group of undergraduates from different majors including Biomedical
composed of a group of undergraduates from different majors including Biomedical
Engineering, Agricultural and Biological Engineering, Biochemistry, and
Engineering, Agricultural and Biological Engineering, Biochemistry, and
-
Chemistry. Bacterial cells such as escherichia coli (E. coli) will be used to
+
Chemistry. Escherichia coli (E. coli) is used to conduct the microbial warfare. The design is to use two different
-
conduct the microbial warfare. The current design includes using three different
+
types of transformed E. coli. One type of E. coli produces protein that triggers the death gene of its opponent (but not for itself) which expresses the production of a protein toxin. One expresses green fluorescence protein and the other expresses red fluorescence protein so that the progression of the war
-
types of transformed E. coli that will compete using unique types of 'kill'
+
between types can be easily viewed and monitered. The team decided the general topic of a bacteria wafare at the end of spring 07 and began to design and construct the wafare in summer 07.
-
mechanisms to overcome the other types of E. coli. Different medias will be
+
-
applied to vary the result of the warfare. Each type of bacteria will also be
+
-
transformed to display a different color so that the progression of the war
+
-
between types can be easily viewed and monitered. The team is excited to be
+
-
working on this project and is moving steadily further to make this project a
+
-
success.
+
==Contact List==
==Contact List==

Revision as of 13:59, 26 October 2007

Contents

Abstract

This year's Purdue 2007 iGEM team will be presenting a project called "Bacteria Warfare" for this year's competition hosted by MIT in November. The team is composed of a group of undergraduates from different majors including Biomedical Engineering, Agricultural and Biological Engineering, Biochemistry, and Chemistry. Escherichia coli (E. coli) is used to conduct the microbial warfare. The design is to use two different types of transformed E. coli. One type of E. coli produces protein that triggers the death gene of its opponent (but not for itself) which expresses the production of a protein toxin. One expresses green fluorescence protein and the other expresses red fluorescence protein so that the progression of the war between types can be easily viewed and monitered. The team decided the general topic of a bacteria wafare at the end of spring 07 and began to design and construct the wafare in summer 07.

Contact List

Faculty Advisors

  • Dr. Rickus rickus@purdue.edu
  • Dr. Clase
  • Dr. Mosier

Graduate Advisors:

Undergraduate Students

Useful References

[http://intl-mmbr.asm.org/cgi/reprint/64/3/503 Programmed Cell Death in Bacteria]

[http://www.springerlink.com/content/n88p472061740k32/fulltext.pdf Spiteful Bacteria ... Who wins?]

[http://jb.asm.org/cgi/reprint/94/4/1093 colicin tolerant E coli mutants]

[http://www.nature.com/nature/journal/v428/n6981/pdf/nature02429.pdf Bacterial Rock Paper Scissors]

Safety

upcoming training

If you have not completed safety training in the last 12 months then please attend the BMED safety training on May 24, 1:30 - 3:30 PM Thursday in BMED 2001.

log your safety training

Work in Lab and should have already compeleted safety training:

  • Meng Zhou
  • Dan Sheik

Bindley Safety

Finally, for safety information specific to the labs in Bindley see the [http://openwetware.org/wiki/Rickus_Lab_Safety Rickus Lab Safety] page on openwetware

Project Description and Details

Photo Area

Our home building on Campus :

[http://www.cyto.purdue.edu/flowcyt/staffpgs/bindly%20for%20ppt.jpg Bindley Bioscience Center]