Template:BerkiGEM2007 VinniConstructionFiles

From 2007.igem.org

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PCR otsAF/otsAIR on otsA     (413 bp, gp=A)
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[[otsA-I716301]]<br>
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PCR otsAIF/otsAR on otsA    (1077 bp, gp=B)
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[[otsB-I716302]]<br>
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--------------------------------------------
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[[otsBA-I716303]]<br>
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PCR otsAF/otsAR on A+B      (1456 bp BglII/XhoI)
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[[otsBA-TT I716307]]<br>
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Digest pBca9145-Bca1144      (BglII/XhoI 2054+910, L)
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[[T7-RBS I716306]]<br>
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Product pBca9145-BBa I716301
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[[otsB-RBS I716305]]<br>
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--------------------------------------------
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[[otsA-TT I716304]]<br>
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otsAF forward BglII site anneals to otsA cgtaaAGATCTATGAGTCGTTTAGTCGTAGTATC
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[[otsB-RBS-otsA-TT I716308]]<br>
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otsAIF Removing BamHI site in otsA CGATGACATTATCTGGATtCACGATTATCACCTG
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[[otsBA Operon I716310]]<br>
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otsAIR Removing BamHI site in otsA CAGGTGATAATCGTGaATCCAGATAATGTCATCG
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[[otsB-otsA Operon I716309]]<br>
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otsaR reverse XhoI site anneals to otsA cgtatCTCGAGttaGGATCCTTACGCAAGCTTTGGAAAGGTAGC
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Latest revision as of 21:09, 30 July 2007

otsA-I716301
otsB-I716302
otsBA-I716303
otsBA-TT I716307
T7-RBS I716306
otsB-RBS I716305
otsA-TT I716304
otsB-RBS-otsA-TT I716308
otsBA Operon I716310
otsB-otsA Operon I716309

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