From 2007.igem.org

Contents 1 Hill function fitting 2 Preliminary assays 2.1 AHL preparation 2.2 Experimental precision and accurecy 3 Assays for hybrid promoter 3.1 Repression and activation check with LacI and AHL 3.2 IPTG assay 3.3 Activation check by cell-produced AHL

Hill function fitting

新しく作ったハイブリッドプロモーターに関してはデータがないので，実験によりパラメータを求める． ヒル関数をfittingさせることにより，n2,n3,K2,K3が求まる. We have obtained data for our newly devised hybrid promoter. By Hill function fitting, we have determined n2, n3, K2, and K3.

導出に関してはfor more detail.

Preliminary assays

Preliminaly assays were done
- to practice and experience the assays with AHL and IPTG
- to check if and how our materials actually worked.

AHL preparation

Experimental precision and accurecy

Assays for hybrid promoter

These assays were done
-to determine the characteristics of our hybrid promoter.
The data from here were used for computational analyses and simulations.

Repression and activation check with LacI and AHL

IPTG assay

Activation check by cell-produced AHL