Tokyo/Preliminary assays

From 2007.igem.org

(Difference between revisions)
 
(3 intermediate revisions not shown)
Line 1: Line 1:
__NOTOC__
__NOTOC__
-
<br>[[Tokyo/Works|Works top]]  0.[[Tokyo/Works/Hybrid promoter|Hybrid promoter]]  1.[[Tokyo/Works/Formulation |Formulation]]  '''2.[[Tokyo/Works/Assay |Assay1]]'''  3.[[Tokyo/Works/Simulation |Simulation]]  4.[[Tokyo/Works/Assay2 |Assay2]]  5.[[Tokyo/Works/Future works |Future works]]
+
<br>[[Tokyo/Works|Works top]]&nbsp;&nbsp;&nbsp;0.[[Tokyo/Works/Hybrid promoter|Hybrid promoter]]&nbsp;&nbsp;&nbsp;1.[[Tokyo/Works/Formulation |Formulation]]  '''2.[[Tokyo/Works/Assay |Assay1]]'''&nbsp;&nbsp;&nbsp;3.[[Tokyo/Works/Simulation |Simulation]]&nbsp;&nbsp;&nbsp;4.[[Tokyo/Works/Assay2 |Assay2]]&nbsp;&nbsp;&nbsp;5.[[Tokyo/Works/Future works |Future works]]
<br><br>
<br><br>
-
[[Tokyo/Hill function fitting |Objective of this assay]]  [[Tokyo/AHL assay|Effect of AHL]]  [[Tokyo/IPTG assay|Effect of IPTG]]  [[Tokyo/Preliminary assays |Preliminary assays]]
+
[[Tokyo/Hill function fitting |Objective of this assay]]&nbsp;&nbsp;&nbsp;[[Tokyo/AHL assay|Effect of AHL]]&nbsp;&nbsp;&nbsp;[[Tokyo/IPTG assay|Effect of LacI-IPTG]]  [[Tokyo/Preliminary assays |Preliminary assays]]
== ==
== ==
-
'''Preliminaly assays were conducted'''
+
'''Preliminary assays were conducted'''
<br>- '''to practice and experience the assays with AHL and IPTG'''
<br>- '''to practice and experience the assays with AHL and IPTG'''
<br>- '''to check if and how our materials actually worked.'''
<br>- '''to check if and how our materials actually worked.'''
== [[Tokyo/AHL preparation |AHL preparation]] ==
== [[Tokyo/AHL preparation |AHL preparation]] ==
-
== [[Tokyo/Experimental precision and accurecy|Experimental precision and accurecy]] ==
+
== [[Tokyo/Experimental precision and accurecy|Experimental precision and accuracy]] ==
== [[Tokyo/AHL assay practice|AHL assay practice]] ==
== [[Tokyo/AHL assay practice|AHL assay practice]] ==
== [[Tokyo/Lux-lac hybrid promoter activation check|Lux-lac hybrid promoter activation check]] ==
== [[Tokyo/Lux-lac hybrid promoter activation check|Lux-lac hybrid promoter activation check]] ==

Latest revision as of 05:12, 27 October 2007


Works top   0.Hybrid promoter   1.Formulation  2.Assay1   3.Simulation   4.Assay2   5.Future works

Objective of this assay   Effect of AHL   Effect of LacI-IPTG  Preliminary assays

Preliminary assays were conducted
- to practice and experience the assays with AHL and IPTG
- to check if and how our materials actually worked.

AHL preparation

Experimental precision and accuracy

AHL assay practice

Lux-lac hybrid promoter activation check

OD correction

Wash

New AHL check