Tokyo/Preliminary assays

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Fig.1: Exogenously added AHL

Preliminaly assays were done
- to practice and experience the assays with AHL and IPTG
- to check if and how our materials actually worked.

Contents

AHL preparation

Experimental precision and accurecy

AHL assay practice

Lux-lac hybrid promoter activation check

OD correction

Wash

New AHL check

Hybrid promoter activation & repression check