Tokyo/alternative

From 2007.igem.org

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(2-b. Combinational Wet & Dry Approaches)
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===2-a. [[Tokyo/Genetic circuit|Genetic circuits]] ===
===2-a. [[Tokyo/Genetic circuit|Genetic circuits]] ===
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===2-b. Combinational Wet & Dry Approaches===
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===2-b. Combinatione of Wet & Dry===
===0. <span style="color:#ff33cc;">Wet </span>: [[Tokyo/Hybrid promoter|Hybrid promoter]]===
===0. <span style="color:#ff33cc;">Wet </span>: [[Tokyo/Hybrid promoter|Hybrid promoter]]===

Revision as of 15:43, 22 October 2007

Title.JPG

1. Abstruct

Concept & Model

A bacterial society designed in this project follows Pareto's principle as an Ant society does.
To realize this model, the behaviors of the model - bistability of each individual and the system's stability under the coexsistence of the two types - have been confirmed in silico and in virto.
Tokyo simulation s2.jpg
Our goal.JPG

Requirements

Bistability
Coexistence by cell-cell communication.


2. Works

2-a. Genetic circuits

2-b. Combinatione of Wet & Dry

0. Wet : Hybrid promoter

The newly deviced hybrid promoter regulated by two inputs.

Hybridgraph.JPG

1. Dry :  Formulation

2. Wet :  Assay

For the parameters of Hill coefficients by AHL and IPTG assays Hill AHL.JPG|Hill IPTG.JPG

3. Dry :  Simulation

4. Wet :  Assay

We tested whether our plasmids gave the other parameters to satisfy the simulation by the experiments using E. coli.

5. Future works

Continue this combinational approaches to sophisticate our model. See more.


3. About our team

Students
Satoru Akama | Kenichiro Iwasaki | Hiroki Kawahara | Hajime Nakatani

Advisors: Last year's member
Shotaro Ayukawa | Akio Kobayashi

Instructors
[http://www.sb.dis.titech.ac.jp/ Daisuke Kiga] | [http://www.es.dis.titech.ac.jp/ Masayuki Yamamura] | [http://nicosia.is.s.u-tokyo.ac.jp/~hagiya/ Masami Hagiya] | [http://www.sb.dis.titech.ac.jp/ Masahiko Uchiyama]

Links sunaba