Tokyo/sunaba

From 2007.igem.org

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(We need to develop the promoter that can receive two input,AHL & reperssor.We tryed to create and successed!!)
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For detailed description of each section, plese click "more infromation"
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<h1> ''' 1,[[Tokyo/Project |Project]]''' </h1>
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<h1> ''' 1,[[Tokyo/Project |Project summary]]''' </h1>
===[[Tokyo/Concept |Concept& model]]: Bacterial society that follows Pareto's principle like ant society===
===[[Tokyo/Concept |Concept& model]]: Bacterial society that follows Pareto's principle like ant society===
<!-- <br>[[Image:Ant society.JPG|350px]]-->
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===[[Tokyo/Genetic circuit|Genetic circuit]]:Quorum Sensing===
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===[[Tokyo/Genetic circuit|Genetic circuit with new part]]:===
<br>[[Image:Tokyo_genetic circuit_s.jpg|350px]]
<br>[[Image:Tokyo_genetic circuit_s.jpg|350px]]
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====Our construction parts are [[Tokyo/construction|Here]]!!! ====
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===[[Tokyo/simulation|Simulation]]:using parameters from our wet experiments ===
===[[Tokyo/simulation|Simulation]]:using parameters from our wet experiments ===
<br>[[Image:Tokyo_simulation_s2.jpg|350px]]
<br>[[Image:Tokyo_simulation_s2.jpg|350px]]
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====Our construction parts are [[Tokyo/construction|Here]]!!! ====
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====We need to develop the promoter that can receive two input,AHL & reperssor.We tryed to create and [[Tokyo/Hybrid promoter|successed]]!! ====
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====For our circuit, we developped the [[Tokyo/Hybrid promoter|promoter]] that can receive two input,AHL & reperssor.====
<!--New part development:Hybrid promoterPBとして、2つの入力を受けるプロモータを開発する必要があった
<!--New part development:Hybrid promoterPBとして、2つの入力を受けるプロモータを開発する必要があった
<br>図 概念図、配列とグラフ図
<br>図 概念図、配列とグラフ図
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===2-1.Dry : [[Tokyo/Formulation |Formulation]]===
===2-1.Dry : [[Tokyo/Formulation |Formulation]]===
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We writed the Formulation
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We wrote the Formulation
<!--リプレッサーA,B,シグナル分子Sの時間変化を式として表し、安定性解析を行った結果、Hill係数が大切であることがわかった
<!--リプレッサーA,B,シグナル分子Sの時間変化を式として表し、安定性解析を行った結果、Hill係数が大切であることがわかった
<br>図 3つの式
<br>図 3つの式

Revision as of 09:18, 21 October 2007

砂場。


Homepage1.JPG

For detailed description of each section, plese click "more infromation"

1,Project summary

Concept& model: Bacterial society that follows Pareto's principle like ant society


TOP.JPG

Requirements:


bistability and cell-cell communication

Genetic circuit with new part:


Tokyo genetic circuit s.jpg

Simulation:using parameters from our wet experiments


Tokyo simulation s2.jpg

Our construction parts are Here!!!

2,Works

Approach:We alternated approach of Wet and Dry.

2-0.Wet : Hybrid promoter

For our circuit, we developped the promoter that can receive two input,AHL & reperssor.

Hybrid.JPG


棒グラフも

2-1.Dry : Formulation

We wrote the Formulation

2-2.Wet : Assay

We decided the parameter of hill coefficients by the assay of AHL & IPTG

Hill AHL.JPG|Hill IPTG.JPG

2-3.Dry : Simulation

wet実験で求められたパラメータを用いて、集団が怠け者の細胞のみとなった場合に、一度不安定化して、続いて働き者と怠け者になれることをStocastic Simulationで確認した。このシステムの挙動に必要な他のパラメータ範囲が判明した。

Tokyo simulation s2.jpg

2-4.Wet : Assay

Simulationを満たすための他のパラメータが現在のプラスミドで可能であるかを、大腸菌を用いた実験で確認した。

2-5.Future works

3,About our team


Students
Satoru Akama | Kenichiro Iwasaki | Hiroki Kawahara | Hajime Nakatani

Advisors: Last year's member
Syotarou Ayukawa | Akio Kobayashi

Instructors
[http://www.sb.dis.titech.ac.jp/ Daisuke Kiga] | [http://www.es.dis.titech.ac.jp/ Masayuki Yamamura] | [http://nicosia.is.s.u-tokyo.ac.jp/~hagiya/ Masami Hagiya] | [http://www.sb.dis.titech.ac.jp/ Masahiko Uchiyama]

Links sunaba


Homepage1.JPG


Project

Concept: Ant society and Pareto's principle
Model : Bacterial society that follows Pareto's principle Homepage3.jpg
Requirements: bistability and cell-cell communication

Experiments

Wet

Construction / Assay

Dry

Analysis / Simulation


Achievements

About our team


Students
Satoru Akama | Kenichiro Iwasaki | Hiroki Kawahara | Hajime Nakatani

Advisors: Last year's member
Syotarou Ayukawa | Akio Kobayashi

Instructors
[http://www.sb.dis.titech.ac.jp/ Daisuke Kiga] | [http://www.es.dis.titech.ac.jp/ Masayuki Yamamura] | [http://nicosia.is.s.u-tokyo.ac.jp/~hagiya/ Masami Hagiya] | [http://www.sb.dis.titech.ac.jp/ Masahiko Uchiyama]

Links sunaba

Home https://2007.igem.org/Tokyo_Tech