Tokyo/sunaba2

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====Our construction parts are [[Tokyo/construction|Here]]!!! ====
====Our construction parts are [[Tokyo/construction|Here]]!!! ====
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===[[Tokyo/simulation|Simulation]]:using parameters from our wet experiments ===
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===[[Tokyo/simulation|Our GOAL]]:using parameters from our wet experiments ===
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===2-3.Dry : [[Tokyo/Simulation |Simulation]]===
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===2-4.Wet : [[Tokyo/Assay |Assay]]===
===2-4.Wet : [[Tokyo/Assay |Assay]]===

Revision as of 17:52, 21 October 2007

砂場2 Tokyotech3.JPG

1,Project

Concept & Model

We made bacterial society that follows Pareto's principle,for example ,Ant society


TOP.JPG

Requirements

Our requirements for construction this model are two things,bistability and coexistence by cell-cell communication


Bistability.JPGCoexistence.JPG

Genetic circuit

We constructed the genetic circuit to fill its requirements. Therefore we made new parts


Tokyo genetic circuit s.jpg

Our construction parts are Here!!!

Our GOAL:using parameters from our wet experiments


Tokyo simulation s2.jpg

2,Works


2-0.Wet : Hybrid promoter

Hybridpromoter.JPG Hybridgraph.JPGHybrid.JPG

2-1.Dry : Formulation

Expression4-1.jpg

2-2.Wet : Assay

Hill AHL.JPGHill IPTG.JPG

2-3.Dry : Simulation

2-4.Wet : Assay

Simulationを満たすための他のパラメータが現在のプラスミドで可能であるかを、大腸菌を用いた実験で確認した。

2-5.Future works

3,About our team


Students
Satoru Akama | Kenichiro Iwasaki | Hiroki Kawahara | Hajime Nakatani

Advisors: Last year's member
Syotarou Ayukawa | Akio Kobayashi

Instructors
Daisuke Kiga | Masayuki Yamamura | Masami Hagiya | Masahiko Uchiyama

Links sunaba