Tokyo/sunaba2

From 2007.igem.org

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<br>[[Image:Bistability.JPG|200px]][[Image:Coexistence.JPG|250px]]
<br>[[Image:Bistability.JPG|200px]][[Image:Coexistence.JPG|250px]]
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===[[Tokyo/Genetic circuit|Genetic circuit]]:Quorum Sensing===
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===[[Tokyo/Genetic circuit|Genetic circuit]]===
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====We constructed the genetic circuit to fill its requirements. Therefore we made [[Tokyo/new parts |new parts]] ====
<br>[[Image:Tokyo_genetic circuit_s.jpg|350px]]
<br>[[Image:Tokyo_genetic circuit_s.jpg|350px]]
====Our construction parts are [[Tokyo/construction|Here]]!!! ====
====Our construction parts are [[Tokyo/construction|Here]]!!! ====

Revision as of 16:47, 21 October 2007

砂場2 Tokyotech3.JPG

1,Project

Concept & Model

We made bacterial society that follows Pareto's principle,for example ,Ant society


TOP.JPG

Requirements

Our requirements for construction this model are two things,bistability and coexistence by cell-cell communication


Bistability.JPGCoexistence.JPG

Genetic circuit

We constructed the genetic circuit to fill its requirements. Therefore we made new parts


Tokyo genetic circuit s.jpg

Our construction parts are Here!!!

2,Works

Approach:We alternated approach of Wet and Dry.

2-0.Wet : Hybrid promoter

We need to develop the promoter that can receive two input,AHL & reperssor.We tryed to create and successed!!

Hybrid.JPG

2-1.Dry : Formulation

We writed the Formulation

2-2.Wet : Assay

We decided the parameter of hill coefficients by the assay of AHL & IPTG

Hill AHL.JPG|Hill IPTG.JPG

2-3.Dry : Simulation

wet実験で求められたパラメータを用いて、集団が怠け者の細胞のみとなった場合に、一度不安定化して、続いて働き者と怠け者になれることをStocastic Simulationで確認した。このシステムの挙動に必要な他のパラメータ範囲が判明した。

Tokyo simulation s2.jpg

2-4.Wet : Assay

Simulationを満たすための他のパラメータが現在のプラスミドで可能であるかを、大腸菌を用いた実験で確認した。

2-5.Future works

3,About our team


Students
Satoru Akama | Kenichiro Iwasaki | Hiroki Kawahara | Hajime Nakatani

Advisors: Last year's member
Syotarou Ayukawa | Akio Kobayashi

Instructors
[http://www.sb.dis.titech.ac.jp/ Daisuke Kiga] | [http://www.es.dis.titech.ac.jp/ Masayuki Yamamura] | [http://nicosia.is.s.u-tokyo.ac.jp/~hagiya/ Masami Hagiya] | [http://www.sb.dis.titech.ac.jp/ Masahiko Uchiyama]

Links sunaba