1,Project

Concept & Model

We made bacterial society that follows Pareto's principle,for example ,Ant society

Requirements

Our requirements for construction this model are two things,bistability and coexistence by cell-cell communication

Genetic circuit

We constructed the genetic circuit to fill its requirements. Therefore we made new parts

　　　　　　　　　　　　　　　Our construction parts are Here!!!

Our GOAL

We indicated our goal of the project by the simulation!! The simulation proved that our genetic circuit have existence of solution.

2,Works

Approach:We alternated approach of Wet and Dry.

2-0.Wet : Hybrid promoter

2-1.Dry : Formulation

We writed the Formulation

2-2.Wet : Assay

We decided the parameter of hill coefficients by the assay of AHL & IPTG

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2-3.Dry : Simulation

wet実験で求められたパラメータを用いて、集団が怠け者の細胞のみとなった場合に、一度不安定化して、続いて働き者と怠け者になれることをStocastic Simulationで確認した。このシステムの挙動に必要な他のパラメータ範囲が判明した。

2-4.Wet : Assay

Simulationを満たすための他のパラメータが現在のプラスミドで可能であるかを、大腸菌を用いた実験で確認した。

2-5.Future works

3,About our team

Students
Satoru Akama | Kenichiro Iwasaki | Hiroki Kawahara | Hajime Nakatani

Advisors: Last year's member
Syotarou Ayukawa | Akio Kobayashi

Instructors
Daisuke Kiga | Masayuki Yamamura | Masami Hagiya | Masahiko Uchiyama

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