Virginia Tech/Updates/Phage
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Phage Culture Updates:VT iGEM Project 2007: Engineering an Epidemic |
Introduction: Growing and Working With Bacteriophage LambdaLambda phage is a well-known and extensively used phenomenon in molecular biology. Lambda is a bacteria virus that can take two different pathways after entering a cell through maltose transport proteins. The first and most common pathway is the Lytic pathway. Upon entering the cell, the virus commands the cellular machinery to make new phage particles and, within about 45 minutes, lyses the cell. Alternatively, lambda can enter the Lysogenic pathway, where it incorporates its own genome into the cell's DNA and remains dormant until the right conditions arise (usually involving cellular stress). Lambda's decision is controlled by the lambda promoter:https://static.igem.org/mediawiki/2007/9/96/Lambda_Pr.JPG UpdatesFirst Progress Update Since no one in our lab had worked with phage before, being able to create and use the lysates has been a learning experience. We have relied heavily on Tim Larson, a faculty adviser from Biology, to help us work through the issues we've come across. We are currently working with two types of phage: Lambda ATCC, a wild-type phage, and Lambda EYFP, a fluorescent phage. Here is what we've accomplished so far with each: Lambda ATCC:
Lambda EYFP:
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